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| | ==Crystal structure of a major outer membrane protein from Thermus thermophilus HB27== | | ==Crystal structure of a major outer membrane protein from Thermus thermophilus HB27== |
| - | <StructureSection load='3dzm' size='340' side='right' caption='[[3dzm]], [[Resolution|resolution]] 2.80Å' scene=''> | + | <StructureSection load='3dzm' size='340' side='right'caption='[[3dzm]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3dzm]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet2 Thet2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DZM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DZM FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3dzm]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB27 Thermus thermophilus HB27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DZM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DZM FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.801Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TT_C0834 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=262724 THET2])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dzm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dzm OCA], [http://pdbe.org/3dzm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3dzm RCSB], [http://www.ebi.ac.uk/pdbsum/3dzm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3dzm ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dzm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dzm OCA], [https://pdbe.org/3dzm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dzm RCSB], [https://www.ebi.ac.uk/pdbsum/3dzm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dzm ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q72JD8_THET2 Q72JD8_THET2] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Thet2]] | + | [[Category: Large Structures]] |
| - | [[Category: Brosig, A]]
| + | [[Category: Thermus thermophilus HB27]] |
| - | [[Category: Diederichs, K]]
| + | [[Category: Brosig A]] |
| - | [[Category: Beta-barrel]]
| + | [[Category: Diederichs K]] |
| - | [[Category: Omp]]
| + | |
| - | [[Category: Thermus thermophilus hb27]] | + | |
| - | [[Category: Ttoa]] | + | |
| - | [[Category: Unknown function]] | + | |
| Structural highlights
Function
Q72JD8_THET2
Publication Abstract from PubMed
The thermophilic eubacterium Thermus thermophilus belongs to one of the oldest branches of evolution and has a multilayered cell envelope that differs from that of modern Gram-negative bacteria. Its outer membrane contains integral outer membrane proteins (OMPs), of which only a few are characterized. TtoA, a new beta-barrel OMP, was identified by searching the genome sequence of strain HB27 for the presence of a C-terminal signature sequence. The structure of TtoA was determined to a resolution of 2.8 A, representing the first crystal structure of an OMP from a thermophilic bacterium. TtoA consists of an eight-stranded beta-barrel with a large extracellular part to which a divalent cation is bound. A five-stranded extracellular beta-sheet protrudes out of the membrane-embedded transmembrane barrel and is stabilized by a disulfide bridge. The edge of this beta-sheet forms crystal contacts that could mimic interactions with other proteins. In modern Gram-negative bacteria, the C-terminal signature sequence of OMPs is required for binding to an Omp85 family protein as a prerequisite for its assembly. We present hints that a similar assembly pathway exists in T. thermophilus by an in vitro binding assay, where unfolded TtoA binds to the Thermus Omp85 family protein TtOmp85, while a mutant without the signature sequence does not.
Crystal structure of a major outer membrane protein from Thermus thermophilus HB27.,Brosig A, Nesper J, Boos W, Welte W, Diederichs K J Mol Biol. 2009 Feb 6;385(5):1445-55. Epub 2008 Dec 10. PMID:19101566[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Brosig A, Nesper J, Boos W, Welte W, Diederichs K. Crystal structure of a major outer membrane protein from Thermus thermophilus HB27. J Mol Biol. 2009 Feb 6;385(5):1445-55. Epub 2008 Dec 10. PMID:19101566 doi:10.1016/j.jmb.2008.12.003
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