4kss

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==Crystal Structure of Vibrio cholerae ATPase GspsE Hexamer==
==Crystal Structure of Vibrio cholerae ATPase GspsE Hexamer==
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<StructureSection load='4kss' size='340' side='right' caption='[[4kss]], [[Resolution|resolution]] 7.58&Aring;' scene=''>
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<StructureSection load='4kss' size='340' side='right'caption='[[4kss]], [[Resolution|resolution]] 7.58&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4kss]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseab Pseab]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KSS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KSS FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4kss]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_UCBPP-PA14 Pseudomonas aeruginosa UCBPP-PA14] and [https://en.wikipedia.org/wiki/Vibrio_cholerae_O1_biovar_El_Tor_str._N16961 Vibrio cholerae O1 biovar El Tor str. N16961]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KSS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KSS FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ksr|4ksr]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 7.58&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">epsE, VC_2732, PA14_01030 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208963 PSEAB])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kss OCA], [https://pdbe.org/4kss PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kss RCSB], [https://www.ebi.ac.uk/pdbsum/4kss PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kss ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kss OCA], [http://pdbe.org/4kss PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kss RCSB], [http://www.ebi.ac.uk/pdbsum/4kss PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kss ProSAT]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/GSPE_VIBCH GSPE_VIBCH]] Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins. Required for secretion of cholera toxin through the outer membrane.
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[https://www.uniprot.org/uniprot/A0A0H2ZJ24_PSEAB A0A0H2ZJ24_PSEAB] [https://www.uniprot.org/uniprot/GSPE_VIBCH GSPE_VIBCH] Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins. Required for secretion of cholera toxin through the outer membrane.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The type II secretion system (T2SS), a multiprotein machinery spanning two membranes in Gram-negative bacteria, is responsible for the secretion of folded proteins from the periplasm across the outer membrane. The critical multidomain T2SS assembly ATPase GspEEpsE had not been structurally characterized as a hexamer. Here, four hexamers of Vibrio cholerae GspEEpsE are obtained when fused to Hcp1 as an assistant hexamer, as shown with native mass spectrometry. The enzymatic activity of the GspEEpsE-Hcp1 fusions is approximately 20 times higher than that of a GspEEpsE monomer, indicating that increasing the local concentration of GspEEpsE by the fusion strategy was successful. Crystal structures of GspEEpsE-Hcp1 fusions with different linker lengths reveal regular and elongated hexamers of GspEEpsE with major differences in domain orientation within subunits, and in subunit assembly. SAXS studies on GspEEpsE-Hcp1 fusions suggest that even further variability in GspEEpsE hexamer architecture is likely.
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Hexamers of the Type II Secretion ATPase GspE from Vibrio cholerae with Increased ATPase Activity.,Lu C, Turley S, Marionni ST, Park YJ, Lee KK, Patrick M, Shah R, Sandkvist M, Bush MF, Hol WG Structure. 2013 Aug 13. pii: S0969-2126(13)00256-6. doi:, 10.1016/j.str.2013.06.027. PMID:23954505<ref>PMID:23954505</ref>
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==See Also==
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*[[General secretion pathway protein 3D structures|General secretion pathway protein 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4kss" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Pseab]]
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[[Category: Large Structures]]
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[[Category: Hol, W G]]
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[[Category: Pseudomonas aeruginosa UCBPP-PA14]]
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[[Category: Lu, C Y]]
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[[Category: Vibrio cholerae O1 biovar El Tor str. N16961]]
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[[Category: Park, Y J]]
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[[Category: Hol WG]]
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[[Category: Turley, S]]
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[[Category: Lu CY]]
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[[Category: Epse]]
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[[Category: Park YJ]]
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[[Category: Hexamer]]
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[[Category: Turley S]]
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[[Category: Protein transport]]
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[[Category: Quasi c6 cyclic symmetry]]
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[[Category: T2ss secretion atpase]]
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Current revision

Crystal Structure of Vibrio cholerae ATPase GspsE Hexamer

PDB ID 4kss

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