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| | ==Crystal structure of monomeric photosensitizing fluorescent protein, Supernova== | | ==Crystal structure of monomeric photosensitizing fluorescent protein, Supernova== |
| - | <StructureSection load='3wck' size='340' side='right' caption='[[3wck]], [[Resolution|resolution]] 2.30Å' scene=''> | + | <StructureSection load='3wck' size='340' side='right'caption='[[3wck]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3wck]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WCK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WCK FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3wck]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WCK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WCK FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wck FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wck OCA], [http://pdbe.org/3wck PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wck RCSB], [http://www.ebi.ac.uk/pdbsum/3wck PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wck ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wck FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wck OCA], [https://pdbe.org/3wck PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wck RCSB], [https://www.ebi.ac.uk/pdbsum/3wck PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wck ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q2TCH5_9CNID Q2TCH5_9CNID] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Synthetic construct sequences]] | + | [[Category: Large Structures]] |
| - | [[Category: Matsuda, T]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Nagai, T]] | + | [[Category: Matsuda T]] |
| - | [[Category: Sakai, N]] | + | [[Category: Nagai T]] |
| - | [[Category: Takemoto, K]] | + | [[Category: Sakai N]] |
| - | [[Category: Fluorescent protein]]
| + | [[Category: Takemoto K]] |
| - | [[Category: Genetically encoded photosensitizer]]
| + | |
| - | [[Category: Gfp fold]]
| + | |
| - | [[Category: Phototoxicity]]
| + | |
| Structural highlights
Function
Q2TCH5_9CNID
Publication Abstract from PubMed
Chromophore-assisted light inactivation (CALI) is a powerful technique for acute perturbation of biomolecules in a spatio-temporally defined manner in living specimen with reactive oxygen species (ROS). Whereas a chemical photosensitizer including fluorescein must be added to specimens exogenously and cannot be restricted to particular cells or sub-cellular compartments, a genetically-encoded photosensitizer, KillerRed, can be controlled in its expression by tissue specific promoters or subcellular localization tags. Despite of this superiority, KillerRed hasn't yet become a versatile tool because its dimerization tendency prevents fusion with proteins of interest. Here, we report the development of monomeric variant of KillerRed (SuperNova) by direct evolution using random mutagenesis. In contrast to KillerRed, SuperNova in fusion with target proteins shows proper localization. Furthermore, unlike KillerRed, SuperNova expression alone doesn't perturb mitotic cell division. Supernova retains the ability to generate ROS, and hence promote CALI-based functional analysis of target proteins overcoming the major drawbacks of KillerRed.
SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation.,Takemoto K, Matsuda T, Sakai N, Fu D, Noda M, Uchiyama S, Kotera I, Arai Y, Horiuchi M, Fukui K, Ayabe T, Inagaki F, Suzuki H, Nagai T Sci Rep. 2013 Sep 17;3:2629. doi: 10.1038/srep02629. PMID:24043132[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Takemoto K, Matsuda T, Sakai N, Fu D, Noda M, Uchiyama S, Kotera I, Arai Y, Horiuchi M, Fukui K, Ayabe T, Inagaki F, Suzuki H, Nagai T. SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation. Sci Rep. 2013 Sep 17;3:2629. doi: 10.1038/srep02629. PMID:24043132 doi:10.1038/srep02629
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