This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

3n9u

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Current revision

Crystal Structure of the Complex between the 25 kDa Subunit and the 59 kDa Subunit (RRM domain) of Human Cleavage Factor Im

Structural highlights

3n9u is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.92Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CPSF5_HUMAN Component of the cleavage factor Im (CFIm) complex that plays a key role in pre-mRNA 3'-processing. Involved in association with CPSF6 or CPSF7 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. NUDT21/CPSF5 binds to cleavage and polyadenylation RNA substrates. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. May have a role in mRNA export.^[1] ^[2] ^[3] ^[4] ^[5] ^[6]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

↑ Ruegsegger U, Blank D, Keller W. Human pre-mRNA cleavage factor Im is related to spliceosomal SR proteins and can be reconstituted in vitro from recombinant subunits. Mol Cell. 1998 Jan;1(2):243-53. PMID:9659921
↑ Ruegsegger U, Beyer K, Keller W. Purification and characterization of human cleavage factor Im involved in the 3' end processing of messenger RNA precursors. J Biol Chem. 1996 Mar 15;271(11):6107-13. PMID:8626397
↑ Brown KM, Gilmartin GM. A mechanism for the regulation of pre-mRNA 3' processing by human cleavage factor Im. Mol Cell. 2003 Dec;12(6):1467-76. PMID:14690600
↑ Kim S, Yamamoto J, Chen Y, Aida M, Wada T, Handa H, Yamaguchi Y. Evidence that cleavage factor Im is a heterotetrameric protein complex controlling alternative polyadenylation. Genes Cells. 2010 Sep 1;15(9):1003-13. doi: 10.1111/j.1365-2443.2010.01436.x., Epub 2010 Jul 29. PMID:20695905 doi:10.1111/j.1365-2443.2010.01436.x
↑ Yang Q, Gilmartin GM, Doublie S. Structural basis of UGUA recognition by the Nudix protein CFI(m)25 and implications for a regulatory role in mRNA 3' processing. Proc Natl Acad Sci U S A. 2010 Jun 1;107(22):10062-7. Epub 2010 May 17. PMID:20479262
↑ Yang Q, Coseno M, Gilmartin GM, Doublie S. Crystal Structure of a Human Cleavage Factor CFI(m)25/CFI(m)68/RNA Complex Provides an Insight into Poly(A) Site Recognition and RNA Looping. Structure. 2011 Feb 2. PMID:21295486 doi:10.1016/j.str.2010.12.021

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3n9u"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of the Complex between the 25 kDa Subunit and the 59 kDa Subunit (RRM domain) of Human Cleavage Factor Im==
-<StructureSection load='3n9u' size='340' side='right' caption='[[3n9u]], [[Resolution|resolution]] 1.92&Aring;' scene=''>
+<StructureSection load='3n9u' size='340' side='right'caption='[[3n9u]], [[Resolution|resolution]] 1.92&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3n9u]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N9U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N9U FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3n9u]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N9U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N9U FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.92&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CFIM25, CPSF25, CPSF5, NUDT21 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CPSF7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n9u OCA], [http://pdbe.org/3n9u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3n9u RCSB], [http://www.ebi.ac.uk/pdbsum/3n9u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3n9u ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n9u OCA], [https://pdbe.org/3n9u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n9u RCSB], [https://www.ebi.ac.uk/pdbsum/3n9u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n9u ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/CPSF5_HUMAN CPSF5_HUMAN]] Component of the cleavage factor Im (CFIm) complex that plays a key role in pre-mRNA 3'-processing. Involved in association with CPSF6 or CPSF7 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. NUDT21/CPSF5 binds to cleavage and polyadenylation RNA substrates. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. May have a role in mRNA export.<ref>PMID:9659921</ref> <ref>PMID:8626397</ref> <ref>PMID:14690600</ref> <ref>PMID:20695905</ref> <ref>PMID:20479262</ref> <ref>PMID:21295486</ref>  [[http://www.uniprot.org/uniprot/CPSF7_HUMAN CPSF7_HUMAN]] Component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. Binds to cleavage and polyadenylation RNA substrates.<ref>PMID:8626397</ref>
+[https://www.uniprot.org/uniprot/CPSF5_HUMAN CPSF5_HUMAN] Component of the cleavage factor Im (CFIm) complex that plays a key role in pre-mRNA 3'-processing. Involved in association with CPSF6 or CPSF7 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. NUDT21/CPSF5 binds to cleavage and polyadenylation RNA substrates. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. May have a role in mRNA export.<ref>PMID:9659921</ref> <ref>PMID:8626397</ref> <ref>PMID:14690600</ref> <ref>PMID:20695905</ref> <ref>PMID:20479262</ref> <ref>PMID:21295486</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n9/3n9u_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n9/3n9u_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 24: / Line 24: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
-[[Category: Arrowsmith, C H]]
+[[Category: Large Structures]]
-[[Category: Berg, S van der]]
+[[Category: Arrowsmith CH]]
-[[Category: Berglund, H]]
+[[Category: Berglund H]]
-[[Category: Bountra, C]]
+[[Category: Bountra C]]
-[[Category: Collins, R]]
+[[Category: Collins R]]
-[[Category: Edwards, A M]]
+[[Category: Edwards AM]]
-[[Category: Flodin, S]]
+[[Category: Flodin S]]
-[[Category: Flores, A]]
+[[Category: Flores A]]
-[[Category: Graslund, S]]
+[[Category: Graslund S]]
-[[Category: Hammarstrom, M]]
+[[Category: Hammarstrom M]]
-[[Category: Johansson, I]]
+[[Category: Johansson I]]
-[[Category: Karlberg, T]]
+[[Category: Karlberg T]]
-[[Category: Kol, S]]
+[[Category: Kol S]]
-[[Category: Kotenyova, T]]
+[[Category: Kotenyova T]]
-[[Category: Moche, M]]
+[[Category: Moche M]]
-[[Category: Nielsen, T N]]
+[[Category: Nielsen TN]]
-[[Category: Nordlund, P]]
+[[Category: Nordlund P]]
-[[Category: Nyman, T]]
+[[Category: Nyman T]]
-[[Category: Persson, C]]
+[[Category: Persson C]]
-[[Category: Structural genomic]]
+[[Category: Schuler H]]
-[[Category: Schuler, H]]
+[[Category: Schutz P]]
-[[Category: Schutz, P]]
+[[Category: Siponen MI]]
-[[Category: Siponen, M I]]
+[[Category: Thorsell AG]]
-[[Category: Thorsell, A G]]
+[[Category: Tresaugues L]]
-[[Category: Tresaugues, L]]
+[[Category: Wahlberg E]]
-[[Category: Wahlberg, E]]
+[[Category: Weigelt J]]
-[[Category: Weigelt, J]]
+[[Category: Welin M]]
-[[Category: Welin, M]]
+[[Category: Wisniewska M]]
-[[Category: Wisniewska, M]]
+[[Category: Van der Berg S]]
-[[Category: Cleavage and polyadenylation specificity factor subunit 5]]
-[[Category: Cleavage and polyadenylation specificity factor subunit 7]]
-[[Category: Coexpression]]
-[[Category: Cpsf5]]
-[[Category: Cpsf7]]
-[[Category: Heterotetramer]]
-[[Category: Hydrolase]]
-[[Category: Mrna cleavage]]
-[[Category: Mrna maturation]]
-[[Category: Nudix]]
-[[Category: Nudt21]]
-[[Category: Polyadenylation]]
-[[Category: Pre-mrna cleavage factor im 25 kda subunit]]
-[[Category: Pre-mrna cleavage factor im 59 kda subunit]]
-[[Category: Protein-protein complex]]
-[[Category: Rrm domain]]
-[[Category: Sgc]]
-[[Category: Sgc stockholm]]
-[[Category: Splicing]]

3n9u

From Proteopedia

Current revision

Crystal Structure of the Complex between the 25 kDa Subunit and the 59 kDa Subunit (RRM domain) of Human Cleavage Factor Im

Structural highlights

Function

Evolutionary Conservation

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox