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| | ==Structure of Full-length Drosophila Cryptochrome== | | ==Structure of Full-length Drosophila Cryptochrome== |
| - | <StructureSection load='4gu5' size='340' side='right' caption='[[4gu5]], [[Resolution|resolution]] 2.30Å' scene=''> | + | <StructureSection load='4gu5' size='340' side='right'caption='[[4gu5]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4gu5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3tvs 3tvs]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GU5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GU5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4gu5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3tvs 3tvs]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GU5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GU5 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CG3772, cry ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gu5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gu5 OCA], [http://pdbe.org/4gu5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gu5 RCSB], [http://www.ebi.ac.uk/pdbsum/4gu5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gu5 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gu5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gu5 OCA], [https://pdbe.org/4gu5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gu5 RCSB], [https://www.ebi.ac.uk/pdbsum/4gu5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gu5 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CRY1_DROME CRY1_DROME]] Blue light-dependent regulator that is the input of the circadian feedback loop. Has no photolyase activity for cyclobutane pyrimidine dimers or 6-4 photoproducts. Regulation of expression by light suggests a role in photoreception for locomotor activity rhythms. Functions, together with per, as a transcriptional repressor required for the oscillation of peripheral circadian clocks and for the correct specification of clock cells. Genes directly activated by the transcription factors Clock (Clk) and cycle (cyc) are repressed by cry. Necessary for light-dependent magnetosensitivity, an intact circadian system is not required for the magnetoreception mechanism to operate. Required for both the naive and trained responses to magnetic field, consistent with the notion that cry is in the input pathway of magnetic sensing.<ref>PMID:9845369</ref> <ref>PMID:10063806</ref> <ref>PMID:9845370</ref> <ref>PMID:10233998</ref> <ref>PMID:10417378</ref> <ref>PMID:16527739</ref> <ref>PMID:17298948</ref> <ref>PMID:18641630</ref> <ref>PMID:18597555</ref> | + | [https://www.uniprot.org/uniprot/CRY1_DROME CRY1_DROME] Blue light-dependent regulator that is the input of the circadian feedback loop. Has no photolyase activity for cyclobutane pyrimidine dimers or 6-4 photoproducts. Regulation of expression by light suggests a role in photoreception for locomotor activity rhythms. Functions, together with per, as a transcriptional repressor required for the oscillation of peripheral circadian clocks and for the correct specification of clock cells. Genes directly activated by the transcription factors Clock (Clk) and cycle (cyc) are repressed by cry. Necessary for light-dependent magnetosensitivity, an intact circadian system is not required for the magnetoreception mechanism to operate. Required for both the naive and trained responses to magnetic field, consistent with the notion that cry is in the input pathway of magnetic sensing.<ref>PMID:9845369</ref> <ref>PMID:10063806</ref> <ref>PMID:9845370</ref> <ref>PMID:10233998</ref> <ref>PMID:10417378</ref> <ref>PMID:16527739</ref> <ref>PMID:17298948</ref> <ref>PMID:18641630</ref> <ref>PMID:18597555</ref> |
| | + | |
| | + | ==See Also== |
| | + | *[[Cryptochrome 3D structures|Cryptochrome 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Drome]] | + | [[Category: Drosophila melanogaster]] |
| - | [[Category: Crane, B R]] | + | [[Category: Large Structures]] |
| - | [[Category: Jones, A R]] | + | [[Category: Crane BR]] |
| - | [[Category: Levy, C]] | + | [[Category: Jones AR]] |
| - | [[Category: Leys, D]] | + | [[Category: Levy C]] |
| - | [[Category: Scrutton, N S]] | + | [[Category: Leys D]] |
| - | [[Category: Top, D]] | + | [[Category: Scrutton NS]] |
| - | [[Category: Vaidya, A T]] | + | [[Category: Top D]] |
| - | [[Category: Widom, J]] | + | [[Category: Vaidya AT]] |
| - | [[Category: Young, M W]] | + | [[Category: Widom J]] |
| - | [[Category: Zoltowski, B D]] | + | [[Category: Young MW]] |
| - | [[Category: Circadian clock light entrainment]]
| + | [[Category: Zoltowski BD]] |
| - | [[Category: Gene regulation]]
| + | |
| - | [[Category: Jetlag]]
| + | |
| - | [[Category: Phosphorylation]]
| + | |
| - | [[Category: Photolyase]]
| + | |
| - | [[Category: Protein degradation]]
| + | |
| - | [[Category: Signaling protein]]
| + | |
| - | [[Category: Timeless]]
| + | |
| Structural highlights
Function
CRY1_DROME Blue light-dependent regulator that is the input of the circadian feedback loop. Has no photolyase activity for cyclobutane pyrimidine dimers or 6-4 photoproducts. Regulation of expression by light suggests a role in photoreception for locomotor activity rhythms. Functions, together with per, as a transcriptional repressor required for the oscillation of peripheral circadian clocks and for the correct specification of clock cells. Genes directly activated by the transcription factors Clock (Clk) and cycle (cyc) are repressed by cry. Necessary for light-dependent magnetosensitivity, an intact circadian system is not required for the magnetoreception mechanism to operate. Required for both the naive and trained responses to magnetic field, consistent with the notion that cry is in the input pathway of magnetic sensing.[1] [2] [3] [4] [5] [6] [7] [8] [9]
See Also
References
- ↑ Emery P, So WV, Kaneko M, Hall JC, Rosbash M. CRY, a Drosophila clock and light-regulated cryptochrome, is a major contributor to circadian rhythm resetting and photosensitivity. Cell. 1998 Nov 25;95(5):669-79. PMID:9845369
- ↑ Okano S, Kanno S, Takao M, Eker AP, Isono K, Tsukahara Y, Yasui A. A putative blue-light receptor from Drosophila melanogaster. Photochem Photobiol. 1999 Jan;69(1):108-13. PMID:10063806
- ↑ Stanewsky R, Kaneko M, Emery P, Beretta B, Wager-Smith K, Kay SA, Rosbash M, Hall JC. The cryb mutation identifies cryptochrome as a circadian photoreceptor in Drosophila. Cell. 1998 Nov 25;95(5):681-92. PMID:9845370
- ↑ Egan ES, Franklin TM, Hilderbrand-Chae MJ, McNeil GP, Roberts MA, Schroeder AJ, Zhang X, Jackson FR. An extraretinally expressed insect cryptochrome with similarity to the blue light photoreceptors of mammals and plants. J Neurosci. 1999 May 15;19(10):3665-73. PMID:10233998
- ↑ Ceriani MF, Darlington TK, Staknis D, Mas P, Petti AA, Weitz CJ, Kay SA. Light-dependent sequestration of TIMELESS by CRYPTOCHROME. Science. 1999 Jul 23;285(5427):553-6. PMID:10417378
- ↑ Collins B, Mazzoni EO, Stanewsky R, Blau J. Drosophila CRYPTOCHROME is a circadian transcriptional repressor. Curr Biol. 2006 Mar 7;16(5):441-9. PMID:16527739 doi:S0960-9822(06)01043-8
- ↑ Berndt A, Kottke T, Breitkreuz H, Dvorsky R, Hennig S, Alexander M, Wolf E. A novel photoreaction mechanism for the circadian blue light photoreceptor Drosophila cryptochrome. J Biol Chem. 2007 Apr 27;282(17):13011-21. Epub 2007 Feb 12. PMID:17298948 doi:M608872200
- ↑ Gegear RJ, Casselman A, Waddell S, Reppert SM. Cryptochrome mediates light-dependent magnetosensitivity in Drosophila. Nature. 2008 Aug 21;454(7207):1014-8. doi: 10.1038/nature07183. Epub 2008 Jul 20. PMID:18641630 doi:10.1038/nature07183
- ↑ Hoang N, Schleicher E, Kacprzak S, Bouly JP, Picot M, Wu W, Berndt A, Wolf E, Bittl R, Ahmad M. Human and Drosophila cryptochromes are light activated by flavin photoreduction in living cells. PLoS Biol. 2008 Jul 1;6(7):e160. doi: 10.1371/journal.pbio.0060160. PMID:18597555 doi:10.1371/journal.pbio.0060160
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