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3vpt

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==Crystal structure of Bombyx mori sigma-class glutathione transferase in apo form==
==Crystal structure of Bombyx mori sigma-class glutathione transferase in apo form==
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<StructureSection load='3vpt' size='340' side='right' caption='[[3vpt]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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<StructureSection load='3vpt' size='340' side='right'caption='[[3vpt]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3vpt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bommo Bommo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VPT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VPT FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3vpt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bombyx_mori Bombyx mori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VPT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VPT FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGO:S-1,2-PROPANEDIOL'>PGO</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3vpq|3vpq]], [[3vpu|3vpu]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGO:S-1,2-PROPANEDIOL'>PGO</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GST sigma ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7091 BOMMO])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vpt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vpt OCA], [https://pdbe.org/3vpt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vpt RCSB], [https://www.ebi.ac.uk/pdbsum/3vpt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vpt ProSAT]</span></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vpt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vpt OCA], [http://pdbe.org/3vpt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vpt RCSB], [http://www.ebi.ac.uk/pdbsum/3vpt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vpt ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q5CCJ4_BOMMO Q5CCJ4_BOMMO]
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BACKGROUND: Glutathione transferases (GSTs) are members of a major family of detoxification enzymes. Here, we report the crystal structure of a sigma-class GST of Bombyx mori, bmGSTS1, to gain insight into the mechanism catalysis. METHODS: The structure of bmGSTS1 and its complex with glutathione were determined at resolutions of 1.9A and 1.7A by synchrotron radiation and the molecular replacement method. RESULTS: The three-dimensional structure of bmGSTS1 shows that it exists as a dimer and is similar in structure to other GSTs with respect to its secondary and tertiary structures. Although striking similarities to the structure of prostaglandin D synthase were also detected, we were surprised to find that bmGSTS1 can convert prostaglandin H2 into its E2 form. Comparison of bmGSTS1 with its glutathione complex showed that bound glutathione was localized to the glutathione-binding site (G-site). Site-directed mutagenesis of bmGSTS1 mutants indicated that amino acid residues Tyr8, Leu14, Trp39, Lys43, Gln50, Met51, Gln63, and Ser64 in the G-site contribute to catalytic activity. CONCLUSION: We determined the tertiary structure of bmGSTS1 exhibiting prostaglandin E synthase activity. GENERAL SIGNIFICANCE: These results are, to our knowledge, the first report of a prostaglandin synthase activity in insects.
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Crystal structure of a Bombyx mori sigma-class glutathione transferase exhibiting prostaglandin E synthase activity.,Yamamoto K, Higashiura A, Suzuki M, Aritake K, Urade Y, Uodome N, Nakagawa A Biochim Biophys Acta. 2013 Jun;1830(6):3711-8. doi: 10.1016/j.bbagen.2013.02.021., Epub 2013 Mar 1. PMID:23458683<ref>PMID:23458683</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3vpt" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[Glutathione S-transferase|Glutathione S-transferase]]
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*[[Glutathione S-transferase 3D structures|Glutathione S-transferase 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bommo]]
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[[Category: Bombyx mori]]
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[[Category: Glutathione transferase]]
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[[Category: Large Structures]]
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[[Category: Higashiura, A]]
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[[Category: Higashiura A]]
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[[Category: Nakagawa, A]]
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[[Category: Nakagawa A]]
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[[Category: Suzuki, M]]
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[[Category: Suzuki M]]
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[[Category: Yamamoto, K]]
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[[Category: Yamamoto K]]
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[[Category: Cytosol]]
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[[Category: Gsh binding]]
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[[Category: Transferase]]
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Current revision

Crystal structure of Bombyx mori sigma-class glutathione transferase in apo form

PDB ID 3vpt

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