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| | ==Clostridium perfringens Enterotoxin at 4.0 Angstrom Resolution== | | ==Clostridium perfringens Enterotoxin at 4.0 Angstrom Resolution== |
| - | <StructureSection load='2yhj' size='340' side='right' caption='[[2yhj]], [[Resolution|resolution]] 4.00Å' scene=''> | + | <StructureSection load='2yhj' size='340' side='right'caption='[[2yhj]], [[Resolution|resolution]] 4.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2yhj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YHJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YHJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2yhj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_perfringens_NCTC_8239 Clostridium perfringens NCTC 8239]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YHJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YHJ FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2xh6|2xh6]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yhj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yhj OCA], [http://pdbe.org/2yhj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2yhj RCSB], [http://www.ebi.ac.uk/pdbsum/2yhj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2yhj ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2yhj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yhj OCA], [https://pdbe.org/2yhj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2yhj RCSB], [https://www.ebi.ac.uk/pdbsum/2yhj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2yhj ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ELTB_CLOPF ELTB_CLOPF]] This enterotoxin is responsible for many cases of a mild type of food poisoning. | + | [https://www.uniprot.org/uniprot/ELTB_CLOPF ELTB_CLOPF] This enterotoxin is responsible for many cases of a mild type of food poisoning. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Clostridium perfringens]] | + | [[Category: Clostridium perfringens NCTC 8239]] |
| - | [[Category: Basak, A K]] | + | [[Category: Large Structures]] |
| - | [[Category: Briggs, D C]] | + | [[Category: Basak AK]] |
| - | [[Category: III, J G.Smedley]] | + | [[Category: Briggs DC]] |
| - | [[Category: McClane, B A]] | + | [[Category: McClane BA]] |
| - | [[Category: Naylor, C E]] | + | [[Category: Naylor CE]] |
| - | [[Category: Antibiotic-associated diarrhoea]] | + | [[Category: Smedley III JG]] |
| - | [[Category: Food-poisoning]]
| + | |
| - | [[Category: Pore-forming toxin]]
| + | |
| - | [[Category: Toxin]]
| + | |
| Structural highlights
Function
ELTB_CLOPF This enterotoxin is responsible for many cases of a mild type of food poisoning.
Publication Abstract from PubMed
Clostridium perfringens enterotoxin (CPE) is a major cause of food poisoning and antibiotic-associated diarrhea. Upon its release from C. perfringens spores, CPE binds to its receptor, claudin, at the tight junctions between the epithelial cells of the gut wall and subsequently forms pores in the cell membranes. A number of different complexes between CPE and claudin have been observed, and the process of pore formation has not been fully elucidated. We have determined the three-dimensional structure of the soluble form of CPE in two crystal forms by X-ray crystallography, to a resolution of 2.7 and 4.0 A, respectively, and found that the N-terminal domain shows structural homology with the aerolysin-like beta-pore-forming family of proteins. We show that CPE forms a trimer in both crystal forms and that this trimer is likely to be biologically relevant but is not the active pore form. We use these data to discuss models of pore formation.
Structure of the Food-Poisoning Clostridium perfringens Enterotoxin Reveals Similarity to the Aerolysin-Like Pore-Forming Toxins.,Briggs DC, Naylor CE, Smedley JG 3rd, Lukoyanova N, Robertson S, Moss DS, McClane BA, Basak AK J Mol Biol. 2011 Oct 14;413(1):138-49. Epub 2011 Aug 3. PMID:21839091[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Briggs DC, Naylor CE, Smedley JG 3rd, Lukoyanova N, Robertson S, Moss DS, McClane BA, Basak AK. Structure of the Food-Poisoning Clostridium perfringens Enterotoxin Reveals Similarity to the Aerolysin-Like Pore-Forming Toxins. J Mol Biol. 2011 Oct 14;413(1):138-49. Epub 2011 Aug 3. PMID:21839091 doi:10.1016/j.jmb.2011.07.066
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