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| | ==Lettuce Necrotic Yellow Virus Phosphoprotein C-Terminal Domain== | | ==Lettuce Necrotic Yellow Virus Phosphoprotein C-Terminal Domain== |
| - | <StructureSection load='3t4r' size='340' side='right' caption='[[3t4r]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='3t4r' size='340' side='right'caption='[[3t4r]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3t4r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lnyv Lnyv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T4R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T4R FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3t4r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lettuce_necrotic_yellows_virus_isolate_318 Lettuce necrotic yellows virus isolate 318]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T4R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T4R FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">P ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=928304 LNYV])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t4r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t4r OCA], [https://pdbe.org/3t4r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t4r RCSB], [https://www.ebi.ac.uk/pdbsum/3t4r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t4r ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t4r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t4r OCA], [http://pdbe.org/3t4r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3t4r RCSB], [http://www.ebi.ac.uk/pdbsum/3t4r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3t4r ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/VP4A_LNYV3 VP4A_LNYV3]] Non catalytic polymerase cofactor and regulatory protein that plays a role in viral transcription and replication. Stabilizes the RNA polymerase L to the N-RNA template and binds the soluble protein N, preventing it from encapsidating non-genomic RNA (By similarity). | + | [https://www.uniprot.org/uniprot/VP4A_LNYV3 VP4A_LNYV3] Non catalytic polymerase cofactor and regulatory protein that plays a role in viral transcription and replication. Stabilizes the RNA polymerase L to the N-RNA template and binds the soluble protein N, preventing it from encapsidating non-genomic RNA (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lnyv]] | + | [[Category: Large Structures]] |
| - | [[Category: Jamin, M]] | + | [[Category: Lettuce necrotic yellows virus isolate 318]] |
| - | [[Category: Martinez, N]] | + | [[Category: Jamin M]] |
| - | [[Category: Tarbouriech, N]] | + | [[Category: Martinez N]] |
| - | [[Category: Helical bundle]] | + | [[Category: Tarbouriech N]] |
| - | [[Category: Nucleoprotein]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| Structural highlights
Function
VP4A_LNYV3 Non catalytic polymerase cofactor and regulatory protein that plays a role in viral transcription and replication. Stabilizes the RNA polymerase L to the N-RNA template and binds the soluble protein N, preventing it from encapsidating non-genomic RNA (By similarity).
Publication Abstract from PubMed
Lettuce necrotic yellows virus (LNYV) is a prototype of the plant-adapted cytorhabdoviruses. Through a meta-prediction of disorder, we localized a folded C-terminal domain in the amino acid sequence of its phosphoprotein. This domain consists of an autonomous folding unit that is monomeric in solution. Its structure, solved by X-ray crystallography, reveals a lollipop-shaped structure comprising five helices. The structure is different from that of the corresponding domains of other Rhabdoviridae, Filoviridae, and Paramyxovirinae; only the overall topology of the polypeptide chain seems to be conserved, suggesting that this domain evolved under weak selective pressure and varied in size by the acquisition or loss of functional modules.
Structure of the C-terminal domain of lettuce necrotic yellows virus phosphoprotein.,Martinez N, Ribeiro EA Jr, Leyrat C, Tarbouriech N, Ruigrok RW, Jamin M J Virol. 2013 Sep;87(17):9569-78. doi: 10.1128/JVI.00999-13. Epub 2013 Jun 19. PMID:23785215[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Martinez N, Ribeiro EA Jr, Leyrat C, Tarbouriech N, Ruigrok RW, Jamin M. Structure of the C-terminal domain of lettuce necrotic yellows virus phosphoprotein. J Virol. 2013 Sep;87(17):9569-78. doi: 10.1128/JVI.00999-13. Epub 2013 Jun 19. PMID:23785215 doi:http://dx.doi.org/10.1128/JVI.00999-13
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