4glf

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==Crystal structure of methylthioadenosine phosphorylase sourced from an antarctic soil metagenomic library==
==Crystal structure of methylthioadenosine phosphorylase sourced from an antarctic soil metagenomic library==
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<StructureSection load='4glf' size='340' side='right' caption='[[4glf]], [[Resolution|resolution]] 1.98&Aring;' scene=''>
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<StructureSection load='4glf' size='340' side='right'caption='[[4glf]], [[Resolution|resolution]] 1.98&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4glf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacterial_sp. Bacterial sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GLF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GLF FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4glf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Uncultured_bacterium Uncultured bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GLF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GLF FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rsfP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=77133 Bacterial sp.])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.98&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4glf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4glf OCA], [http://pdbe.org/4glf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4glf RCSB], [http://www.ebi.ac.uk/pdbsum/4glf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4glf ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4glf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4glf OCA], [https://pdbe.org/4glf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4glf RCSB], [https://www.ebi.ac.uk/pdbsum/4glf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4glf ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/C6KFA4_9BACT C6KFA4_9BACT]] Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.[HAMAP-Rule:MF_01963]
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[https://www.uniprot.org/uniprot/C6KFA4_9BACT C6KFA4_9BACT] Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.[HAMAP-Rule:MF_01963]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The presented study examines the phenomenon of the fluorescence under UV light excitation (312 nm) of E. coli cells expressing a novel metagenomic-derived putative methylthioadenosine phosphorylase gene, called rsfp, grown on LB agar supplemented with a fluorescent dye rhodamine B. For this purpose, an rsfp gene was cloned and expressed in an LMG194 E. coli strain using an arabinose promoter. The resulting RSFP protein was purified and its UV-VIS absorbance spectrum and emission spectrum were assayed. Simultaneously, the same spectroscopic studies were carried out for rhodamine B in the absence or presence of RSFP protein or native E. coli proteins, respectively. The results of the spectroscopic studies suggested that the fluorescence of E. coli cells expressing rsfp gene under UV illumination is due to the interaction of rhodamine B molecules with the RSFP protein. Finally, this interaction was proved by a crystallographic study and then by site-directed mutagenesis of rsfp gene sequence. The crystal structures of RSFP apo form (1.98 A) and complex RSFP/RB (1.90 A) show a trimer of RSFP molecules located on the crystallographic six fold screw axis. The RSFP complex with rhodamine B revealed the binding site for RB, in the pocket located on the interface between symmetry related monomers.
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A study on the interaction of rhodamine B with methylthioadenosine phosphorylase protein sourced from an antarctic soil metagenomic library.,Bartasun P, Cieslinski H, Bujacz A, Wierzbicka-Wos A, Kur J PLoS One. 2013;8(1):e55697. doi: 10.1371/journal.pone.0055697. Epub 2013 Jan 31. PMID:23383268<ref>PMID:23383268</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4glf" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[5'-deoxy-5'-methylthioadenosine phosphorylase|5'-deoxy-5'-methylthioadenosine phosphorylase]]
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*[[5'-deoxy-5'-methylthioadenosine phosphorylase 3D structures|5'-deoxy-5'-methylthioadenosine phosphorylase 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bacterial sp]]
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[[Category: Large Structures]]
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[[Category: Bartasun, P]]
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[[Category: Uncultured bacterium]]
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[[Category: Bujacz, A]]
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[[Category: Bartasun P]]
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[[Category: Bujacz, G]]
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[[Category: Bujacz A]]
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[[Category: Cieslinski, H]]
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[[Category: Bujacz G]]
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[[Category: Metagenomic library antarctic soil]]
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[[Category: Cieslinski H]]
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[[Category: Methylthioadenosine]]
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[[Category: Methylthioadenosine phosphorylase]]
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[[Category: Rhodamine b]]
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[[Category: Transferase]]
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Current revision

Crystal structure of methylthioadenosine phosphorylase sourced from an antarctic soil metagenomic library

PDB ID 4glf

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