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| | ==Crystal Structure of Fischerella Transcription Factor HetR complexed with 21mer DNA target== | | ==Crystal Structure of Fischerella Transcription Factor HetR complexed with 21mer DNA target== |
| - | <StructureSection load='4izz' size='340' side='right' caption='[[4izz]], [[Resolution|resolution]] 2.50Å' scene=''> | + | <StructureSection load='4izz' size='340' side='right'caption='[[4izz]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4izz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Fischerella_pcc7521 Fischerella pcc7521]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IZZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IZZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4izz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Anabaena Anabaena] and [https://en.wikipedia.org/wiki/Fischerella_thermalis_PCC_7521 Fischerella thermalis PCC 7521]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IZZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IZZ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.502Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4j00|4j00]], [[4j01|4j01]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hetR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=98439 Fischerella PCC7521])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4izz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4izz OCA], [https://pdbe.org/4izz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4izz RCSB], [https://www.ebi.ac.uk/pdbsum/4izz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4izz ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4izz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4izz OCA], [http://pdbe.org/4izz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4izz RCSB], [http://www.ebi.ac.uk/pdbsum/4izz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4izz ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q2ACK9_9CYAN Q2ACK9_9CYAN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Fischerella pcc7521]] | + | [[Category: Anabaena]] |
| - | [[Category: Gornicki, P]] | + | [[Category: Fischerella thermalis PCC 7521]] |
| - | [[Category: Joachimiak, A]] | + | [[Category: Large Structures]] |
| - | [[Category: Joachimiak, G]] | + | [[Category: Gornicki P]] |
| - | [[Category: Kim, Y]] | + | [[Category: Joachimiak A]] |
| - | [[Category: Structural genomic]] | + | [[Category: Joachimiak G]] |
| - | [[Category: Mcsg]] | + | [[Category: Kim Y]] |
| - | [[Category: Dna-binding domain]]
| + | [[Category: MCSG]] |
| - | [[Category: Helix-turn-helix]]
| + | |
| - | [[Category: Psi-biology]]
| + | |
| - | [[Category: Transcription factor]]
| + | |
| - | [[Category: Transcription-dna complex]] | + | |
| Structural highlights
Function
Q2ACK9_9CYAN
Publication Abstract from PubMed
HetR is an essential regulator of heterocyst development in cyanobacteria. Many mutations in HetR render Anabaena incapable of nitrogen fixation. The protein binds to a DNA palindrome upstream of hetP and other genes. We have determined the crystal structures of HetR complexed with palindromic DNA targets, 21, 23, and 29 bp at 2.50-, 3.00-, and 3.25-A resolution, respectively. The highest-resolution structure shows fine details of specific protein-DNA interactions. The lower-resolution structures with longer DNA duplexes have similar interaction patterns and show how the flap domains interact with DNA in a sequence nonspecific fashion. Fifteen of 15 protein-DNA contacts predicted on the basis of the structure were confirmed by single amino acid mutations that abolished binding in vitro and complementation in vivo. A striking feature of the structure is the association of glutamate 71 from each subunit of the HetR dimer with three successive cytosines in each arm of the palindromic target, a feature that is conserved among all known heterocyst-forming cyanobacteria sequenced to date.
Structures of complexes comprised of Fischerella transcription factor HetR with Anabaena DNA targets.,Kim Y, Ye Z, Joachimiak G, Videau P, Young J, Hurd K, Callahan SM, Gornicki P, Zhao J, Haselkorn R, Joachimiak A Proc Natl Acad Sci U S A. 2013 Apr 22. PMID:23610410[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kim Y, Ye Z, Joachimiak G, Videau P, Young J, Hurd K, Callahan SM, Gornicki P, Zhao J, Haselkorn R, Joachimiak A. Structures of complexes comprised of Fischerella transcription factor HetR with Anabaena DNA targets. Proc Natl Acad Sci U S A. 2013 Apr 22. PMID:23610410 doi:10.1073/pnas.1305971110
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