|
|
| (One intermediate revision not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Crystal Structure of human splicing factor dim2/TXNL4B== | | ==Crystal Structure of human splicing factor dim2/TXNL4B== |
| - | <StructureSection load='4in0' size='340' side='right' caption='[[4in0]], [[Resolution|resolution]] 1.33Å' scene=''> | + | <StructureSection load='4in0' size='340' side='right'caption='[[4in0]], [[Resolution|resolution]] 1.33Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4in0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IN0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IN0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4in0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IN0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IN0 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SUC:SUCROSE'>SUC</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.327Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DIM2, DLP, TXNL4B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900003:sucrose'>PRD_900003</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4in0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4in0 OCA], [http://pdbe.org/4in0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4in0 RCSB], [http://www.ebi.ac.uk/pdbsum/4in0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4in0 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4in0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4in0 OCA], [https://pdbe.org/4in0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4in0 RCSB], [https://www.ebi.ac.uk/pdbsum/4in0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4in0 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TXN4B_HUMAN TXN4B_HUMAN]] Essential role in pre-mRNA splicing. Required in cell cycle progression for S/G(2) transition.<ref>PMID:15161931</ref> | + | [https://www.uniprot.org/uniprot/TXN4B_HUMAN TXN4B_HUMAN] Essential role in pre-mRNA splicing. Required in cell cycle progression for S/G(2) transition.<ref>PMID:15161931</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 23: |
Line 23: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Guo, F]] | + | [[Category: Large Structures]] |
| - | [[Category: Jin, T C]] | + | [[Category: Guo F]] |
| - | [[Category: Zhang, Y Z]] | + | [[Category: Jin TC]] |
| - | [[Category: Pre-mrna splicing]] | + | [[Category: Zhang YZ]] |
| - | [[Category: Splicing]]
| + | |
| Structural highlights
Function
TXN4B_HUMAN Essential role in pre-mRNA splicing. Required in cell cycle progression for S/G(2) transition.[1]
Publication Abstract from PubMed
TXNL4A (thioredoxin-like 4A) is an essential protein conserved from yeast to humans and is a component of the pre-mRNA splicing machinery. TXNL4B was identified as a TXNL4-family protein that also interacts with Prp6, an integral component of the U4/U6.U5 tri-snRNP complex, and has been shown to function in pre-mRNA splicing. A crystal structure of TXNL4B was determined at 1.33 A resolution and refined to an Rwork of 0.13 and an Rfree of 0.18 with one native dimer in the asymmetric unit. Residues 1-33 of TXNL4B have previously been reported to be responsible for its interaction with Prp6. However, this region extends to the beta-sheet core of the thioredoxin-fold structure of TXNL4B. This suggests that the interpretation of the previously reported GST pull-down results without considering the structure and stability of TXNL4B is debatable.
High-resolution crystal structure of human Dim2/TXNL4B.,Jin T, Guo F, Wang Y, Zhang Y Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Mar 1;69(Pt 3):223-7. doi:, 10.1107/S1744309113000973. Epub 2013 Feb 22. PMID:23519793[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sun X, Zhang H, Wang D, Ma D, Shen Y, Shang Y. DLP, a novel Dim1 family protein implicated in pre-mRNA splicing and cell cycle progression. J Biol Chem. 2004 Jul 30;279(31):32839-47. Epub 2004 May 25. PMID:15161931 doi:10.1074/jbc.M402522200
- ↑ Jin T, Guo F, Wang Y, Zhang Y. High-resolution crystal structure of human Dim2/TXNL4B. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Mar 1;69(Pt 3):223-7. doi:, 10.1107/S1744309113000973. Epub 2013 Feb 22. PMID:23519793 doi:10.1107/S1744309113000973
|
