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| | ==Crystal structure of mEosFP in its green state== | | ==Crystal structure of mEosFP in its green state== |
| - | <StructureSection load='3p8u' size='340' side='right' caption='[[3p8u]], [[Resolution|resolution]] 2.25Å' scene=''> | + | <StructureSection load='3p8u' size='340' side='right'caption='[[3p8u]], [[Resolution|resolution]] 2.25Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3p8u]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lobhe Lobhe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P8U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3P8U FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3p8u]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lobophyllia_hemprichii Lobophyllia hemprichii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P8U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P8U FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO3:SULFITE+ION'>SO3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR8:2-[1-AMINO-2-(1H-IMIDAZOL-5-YL)ETHYL]-1-(CARBOXYMETHYL)-4-[(4-OXOCYCLOHEXA-2,5-DIEN-1-YLIDENE)METHYL]-1H-IMIDAZOL-5-OLATE'>CR8</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CR8:2-[1-AMINO-2-(1H-IMIDAZOL-5-YL)ETHYL]-1-(CARBOXYMETHYL)-4-[(4-OXOCYCLOHEXA-2,5-DIEN-1-YLIDENE)METHYL]-1H-IMIDAZOL-5-OLATE'>CR8</scene>, <scene name='pdbligand=SO3:SULFITE+ION'>SO3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1zux|1zux]], [[2btj|2btj]], [[2vvh|2vvh]], [[2vvi|2vvi]], [[2vvj|2vvj]]</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p8u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p8u OCA], [https://pdbe.org/3p8u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p8u RCSB], [https://www.ebi.ac.uk/pdbsum/3p8u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p8u ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3p8u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p8u OCA], [http://pdbe.org/3p8u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3p8u RCSB], [http://www.ebi.ac.uk/pdbsum/3p8u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3p8u ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q5S6Z9_LOBHE Q5S6Z9_LOBHE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Green Fluorescent Protein|Green Fluorescent Protein]] | + | *[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lobhe]] | + | [[Category: Large Structures]] |
| - | [[Category: Adam, V]] | + | [[Category: Lobophyllia hemprichii]] |
| - | [[Category: Bourgeois, D]] | + | [[Category: Adam V]] |
| - | [[Category: Nienhaus, G U]] | + | [[Category: Bourgeois D]] |
| - | [[Category: Beta-barrel]] | + | [[Category: Nienhaus GU]] |
| - | [[Category: Fluorescent protein]]
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| Structural highlights
Function
Q5S6Z9_LOBHE
Publication Abstract from PubMed
Advanced fluorescence imaging, including subdiffraction microscopy, relies on fluorophores with controllable emission properties. Chief among these fluorophores are the photoactivatable fluorescent proteins capable of reversible on/off photoswitching or irreversible green-to-red photoconversion. IrisFP was recently reported as the first fluorescent protein combining these two types of phototransformations. The introduction of this protein resulted in new applications such as super-resolution pulse-chase imaging. However, the spectroscopic properties of IrisFP are far from being optimal and its tetrameric organization complicates its use as a fusion tag. Here, we demonstrate how four-state optical highlighting can be rationally introduced into photoconvertible fluorescent proteins and develop and characterize a new set of such enhanced optical highlighters derived from mEosFP and Dendra2. We present in particular NijiFP, a promising new fluorescent protein with photoconvertible and biphotochromic properties that make it ideal for advanced fluorescence-based imaging applications.
Rational design of photoconvertible and biphotochromic fluorescent proteins for advanced microscopy applications.,Adam V, Moeyaert B, David CC, Mizuno H, Lelimousin M, Dedecker P, Ando R, Miyawaki A, Michiels J, Engelborghs Y, Hofkens J Chem Biol. 2011 Oct 28;18(10):1241-51. PMID:22035793[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Adam V, Moeyaert B, David CC, Mizuno H, Lelimousin M, Dedecker P, Ando R, Miyawaki A, Michiels J, Engelborghs Y, Hofkens J. Rational design of photoconvertible and biphotochromic fluorescent proteins for advanced microscopy applications. Chem Biol. 2011 Oct 28;18(10):1241-51. PMID:22035793 doi:10.1016/j.chembiol.2011.08.007
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