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4myy

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==Structure of a class 2 docking domain complex from modules CurG and CurH of the curacin A polyketide synthase==
==Structure of a class 2 docking domain complex from modules CurG and CurH of the curacin A polyketide synthase==
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<StructureSection load='4myy' size='340' side='right' caption='[[4myy]], [[Resolution|resolution]] 1.68&Aring;' scene=''>
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<StructureSection load='4myy' size='340' side='right'caption='[[4myy]], [[Resolution|resolution]] 1.68&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4myy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lyngbya_majuscula_3l Lyngbya majuscula 3l]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MYY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MYY FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4myy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Moorena_producens_3L Moorena producens 3L]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MYY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MYY FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.68&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4myz|4myz]], [[4mz0|4mz0]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LYNGBM3L_74500,LYNGBM3L_74480 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=489825 Lyngbya majuscula 3L])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4myy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4myy OCA], [https://pdbe.org/4myy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4myy RCSB], [https://www.ebi.ac.uk/pdbsum/4myy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4myy ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4myy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4myy OCA], [http://pdbe.org/4myy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4myy RCSB], [http://www.ebi.ac.uk/pdbsum/4myy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4myy ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/F4Y428_9CYAN F4Y428_9CYAN] [https://www.uniprot.org/uniprot/F4Y429_9CYAN F4Y429_9CYAN]
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Modular type I polyketide synthases (PKSs) are versatile biosynthetic systems that initiate, successively elongate, and modify acyl chains. Intermediate transfer between modules is mediated via docking domains, which are attractive targets for PKS pathway engineering to produce natural product analogs. We identified a class 2 docking domain in cyanobacterial PKSs and determined crystal structures for two docking domain pairs, revealing a distinct class 2 docking strategy for promoting intermediate transfer. The selectivity of class 2 docking interactions, demonstrated in binding and biochemical assays, could be altered by mutagenesis. We determined the ideal fusion location for exchanging class 1 and class 2 docking domains and demonstrated effective polyketide chain transfer in heterologous modules. Thus, class 2 docking domains are tools for rational bioengineering of a broad range of PKSs containing either class 1 or 2 docking domains.
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Cyanobacterial polyketide synthase docking domains: a tool for engineering natural product biosynthesis.,Whicher JR, Smaga SS, Hansen DA, Brown WC, Gerwick WH, Sherman DH, Smith JL Chem Biol. 2013 Nov 21;20(11):1340-51. doi: 10.1016/j.chembiol.2013.09.015. Epub , 2013 Oct 31. PMID:24183970<ref>PMID:24183970</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4myy" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Lyngbya majuscula 3l]]
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[[Category: Large Structures]]
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[[Category: Smaga, S S]]
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[[Category: Moorena producens 3L]]
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[[Category: Smith, J L]]
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[[Category: Smaga SS]]
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[[Category: Whicher, J R]]
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[[Category: Smith JL]]
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[[Category: Fusion protein]]
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[[Category: Whicher JR]]
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[[Category: Protein binding]]
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[[Category: Protein-protein interaction]]
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Current revision

Structure of a class 2 docking domain complex from modules CurG and CurH of the curacin A polyketide synthase

PDB ID 4myy

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