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3u5y

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==Structures of Alkaloid Biosynthetic Glucosidases Decode Substrate Specificity==
==Structures of Alkaloid Biosynthetic Glucosidases Decode Substrate Specificity==
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<StructureSection load='3u5y' size='340' side='right' caption='[[3u5y]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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<StructureSection load='3u5y' size='340' side='right'caption='[[3u5y]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3u5y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Devilpepper Devilpepper]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U5Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3U5Y FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3u5y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rauvolfia_serpentina Rauvolfia serpentina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U5Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U5Y FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SCG:METHYL+(2S,3R,4S)-2-(BETA-D-GLUCOPYRANOSYLOXY)-4-(2-OXOETHYL)-3-VINYL-3,4-DIHYDRO-2H-PYRAN-5-CARBOXYLATE'>SCG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3u57|3u57]], [[3u5u|3u5u]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SCG:METHYL+(2S,3R,4S)-2-(BETA-D-GLUCOPYRANOSYLOXY)-4-(2-OXOETHYL)-3-VINYL-3,4-DIHYDRO-2H-PYRAN-5-CARBOXYLATE'>SCG</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Raucaffricine_beta-glucosidase Raucaffricine beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.125 3.2.1.125] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u5y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u5y OCA], [https://pdbe.org/3u5y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u5y RCSB], [https://www.ebi.ac.uk/pdbsum/3u5y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u5y ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3u5y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u5y OCA], [http://pdbe.org/3u5y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3u5y RCSB], [http://www.ebi.ac.uk/pdbsum/3u5y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3u5y ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/RG1_RAUSE RG1_RAUSE] Glucosidase specifically involved in alkaloid biosynthesis leading to the accumulation of several alkaloids, including ajmaline, an important plant-derived pharmaceutical used in the treatment of heart disorders.<ref>PMID:10975500</ref>
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Two similar enzymes with different biosynthetic function in one species have evolved to catalyze two distinct reactions. X-ray structures of both enzymes help reveal their most important differences. The Rauvolfia alkaloid biosynthetic network harbors two O-glucosidases: raucaffricine glucosidase (RG), which hydrolyses raucaffricine to an intermediate downstream in the ajmaline pathway, and strictosidine glucosidase (SG), which operates upstream. RG converts strictosidine, the substrate of SG, but SG does not accept raucaffricine. Now elucidation of crystal structures of RG, inactive RG-E186Q mutant, and its complexes with ligands dihydro-raucaffricine and secologanin reveals that it is the "wider gate" of RG that allows strictosidine to enter the catalytic site, whereas the "slot-like" entrance of SG prohibits access by raucaffricine. Trp392 in RG and Trp388 in SG control the gate shape and acceptance of substrates. Ser390 directs the conformation of Trp392. 3D structures, supported by site-directed mutations and kinetic data of RG and SG, provide a structural and catalytic explanation of substrate specificity and deeper insights into O-glucosidase chemistry.
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Structures of Alkaloid Biosynthetic Glucosidases Decode Substrate Specificity.,Xia L, Ruppert M, Wang M, Panjikar S, Lin H, Rajendran C, Barleben L, Stockigt J ACS Chem Biol. 2011 Oct 28. PMID:22004291<ref>PMID:22004291</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3u5y" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[Beta-glucosidase|Beta-glucosidase]]
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*[[Beta-glucosidase 3D structures|Beta-glucosidase 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Devilpepper]]
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[[Category: Large Structures]]
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[[Category: Raucaffricine beta-glucosidase]]
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[[Category: Rauvolfia serpentina]]
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[[Category: Barleben, L]]
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[[Category: Barleben L]]
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[[Category: Lin, H]]
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[[Category: Lin H]]
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[[Category: Panjikar, S]]
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[[Category: Panjikar S]]
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[[Category: Rajendran, C]]
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[[Category: Rajendran C]]
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[[Category: Ruppert, M]]
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[[Category: Ruppert M]]
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[[Category: Stoeckigt, J]]
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[[Category: Stoeckigt J]]
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[[Category: Wang, M]]
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[[Category: Wang M]]
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[[Category: Xia, L]]
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[[Category: Xia L]]
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[[Category: Glucosidase]]
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[[Category: Hydrolase]]
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Current revision

Structures of Alkaloid Biosynthetic Glucosidases Decode Substrate Specificity

PDB ID 3u5y

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