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| | ==Crystal Structure of the Human BTN3A2 Ectodomain== | | ==Crystal Structure of the Human BTN3A2 Ectodomain== |
| - | <StructureSection load='4f8q' size='340' side='right' caption='[[4f8q]], [[Resolution|resolution]] 2.38Å' scene=''> | + | <StructureSection load='4f8q' size='340' side='right'caption='[[4f8q]], [[Resolution|resolution]] 2.38Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4f8q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F8Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F8Q FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4f8q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F8Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F8Q FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BT3.2, BTF3, BTF4, BTN3A2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3789Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f8q OCA], [http://pdbe.org/4f8q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4f8q RCSB], [http://www.ebi.ac.uk/pdbsum/4f8q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4f8q ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f8q OCA], [https://pdbe.org/4f8q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f8q RCSB], [https://www.ebi.ac.uk/pdbsum/4f8q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f8q ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/BT3A2_HUMAN BT3A2_HUMAN]] Plays a role in T-cell responses in the adaptive immune response. Inhibits the release of IFNG from activated T-cells.<ref>PMID:21918970</ref> <ref>PMID:22767497</ref> | + | [https://www.uniprot.org/uniprot/BT3A2_HUMAN BT3A2_HUMAN] Plays a role in T-cell responses in the adaptive immune response. Inhibits the release of IFNG from activated T-cells.<ref>PMID:21918970</ref> <ref>PMID:22767497</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Adams, E J]] | + | [[Category: Large Structures]] |
| - | [[Category: Bonneville, M]] | + | [[Category: Adams EJ]] |
| - | [[Category: Harly, C]] | + | [[Category: Bonneville M]] |
| - | [[Category: Nedellec, S]] | + | [[Category: Harly C]] |
| - | [[Category: Olive, D]] | + | [[Category: Nedellec S]] |
| - | [[Category: Palakodeti, A]] | + | [[Category: Olive D]] |
| - | [[Category: Sandstrom, A]] | + | [[Category: Palakodeti A]] |
| - | [[Category: Scotet, E]] | + | [[Category: Sandstrom A]] |
| - | [[Category: Sundaresan, L]] | + | [[Category: Scotet E]] |
| - | [[Category: B7 superfamily]]
| + | [[Category: Sundaresan L]] |
| - | [[Category: Butyrophilin]]
| + | |
| - | [[Category: Cd277]]
| + | |
| - | [[Category: Immune system]]
| + | |
| Structural highlights
Function
BT3A2_HUMAN Plays a role in T-cell responses in the adaptive immune response. Inhibits the release of IFNG from activated T-cells.[1] [2]
Publication Abstract from PubMed
Human Vgamma9Vdelta2 T cells are well known for their rapid and potent response to infection and tumorigenesis when in the presence of endogenous or exogenous phosphoisoprenoids. However, the molecular mechanisms behind the activation of this gammadelta T cell population remains unclear. Evidence pointing to a role for the CD277/butyrophilin-3 (BTN3A) molecules in this response led us to investigate the structures of these molecules and their modifications upon binding to an agonist antibody (20.1) that mimics phosphoisoprenoid-mediated Vgamma9Vdelta2 activation and an antagonist antibody (103.2) that inhibits this reactivity. We find that the three BTN3A isoforms: BTN3A1, BTN3A2, and BTN3A3, have high structural homology to the B7 superfamily of proteins and exist as V-shaped homodimers in solution, associating through the membrane proximal C-type Ig domain. The 20.1 and 103.2 antibodies bind to separate epitopes on the BTN3A Ig-V domain with high affinity but likely with different valencies based on their binding orientation. These structures directly complement functional studies of this system that demonstrate that BTN3A1 is necessary for Vgamma9Vdelta2 activation and begin to unravel the extracellular events that occur during stimulation through the Vgamma9Vdelta2 T cell receptor.
The molecular basis for modulation of human Vgamma9Vdelta2 T cell responses by CD277/butyrophilin-3 (BTN3A)-specific antibodies.,Palakodeti A, Sandstrom A, Sundaresan L, Harly C, Nedellec S, Olive D, Scotet E, Bonneville M, Adams EJ J Biol Chem. 2012 Sep 21;287(39):32780-90. Epub 2012 Jul 30. PMID:22846996[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Messal N, Mamessier E, Sylvain A, Celis-Gutierrez J, Thibult ML, Chetaille B, Firaguay G, Pastor S, Guillaume Y, Wang Q, Hirsch I, Nunes JA, Olive D. Differential role for CD277 as a co-regulator of the immune signal in T and NK cells. Eur J Immunol. 2011 Dec;41(12):3443-54. doi: 10.1002/eji.201141404. Epub 2011 Nov, 3. PMID:21918970 doi:10.1002/eji.201141404
- ↑ Harly C, Guillaume Y, Nedellec S, Peigne CM, Monkkonen H, Monkkonen J, Li J, Kuball J, Adams EJ, Netzer S, Dechanet-Merville J, Leger A, Herrmann T, Breathnach R, Olive D, Bonneville M, Scotet E. Key implication of CD277/butyrophilin-3 (BTN3A) in cellular stress sensing by a major human gammadelta T-cell subset. Blood. 2012 Sep 13;120(11):2269-79. doi: 10.1182/blood-2012-05-430470. Epub 2012 , Jul 5. PMID:22767497 doi:10.1182/blood-2012-05-430470
- ↑ Palakodeti A, Sandstrom A, Sundaresan L, Harly C, Nedellec S, Olive D, Scotet E, Bonneville M, Adams EJ. The molecular basis for modulation of human Vgamma9Vdelta2 T cell responses by CD277/butyrophilin-3 (BTN3A)-specific antibodies. J Biol Chem. 2012 Sep 21;287(39):32780-90. Epub 2012 Jul 30. PMID:22846996 doi:10.1074/jbc.M112.384354
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