4isr

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==Binding domain of Botulinum neurotoxin DC in complex with rat synaptotagmin II==
==Binding domain of Botulinum neurotoxin DC in complex with rat synaptotagmin II==
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<StructureSection load='4isr' size='340' side='right' caption='[[4isr]], [[Resolution|resolution]] 2.59&Aring;' scene=''>
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<StructureSection load='4isr' size='340' side='right'caption='[[4isr]], [[Resolution|resolution]] 2.59&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4isr]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ISR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ISR FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4isr]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ISR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ISR FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.59&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4isq|4isq]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4isr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4isr OCA], [http://pdbe.org/4isr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4isr RCSB], [http://www.ebi.ac.uk/pdbsum/4isr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4isr ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4isr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4isr OCA], [https://pdbe.org/4isr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4isr RCSB], [https://www.ebi.ac.uk/pdbsum/4isr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4isr ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/SYT2_RAT SYT2_RAT]] May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone.
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[https://www.uniprot.org/uniprot/Q9LBR1_CLOBO Q9LBR1_CLOBO]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Botulinum neurotoxins (BoNTs) can cause paralysis at exceptionally low concentrations and include seven serotypes (BoNT/A-G). The chimeric BoNT/DC toxin has a receptor binding domain similar to the same region in BoNT/C. However, BoNT/DC does not share protein receptor with BoNT/C. Instead, it shares synaptotagmin (Syt) I and II as receptors with BoNT/B, despite their low sequence similarity. Here, we present the crystal structures of the binding domain of BoNT/DC in complex with the recognition domains of its protein receptors, Syt-I and Syt-II. The structures reveal that BoNT/DC possesses a Syt binding site, distinct from the established Syt-II binding site in BoNT/B. Structure-based mutagenesis further shows that hydrophobic interactions play a key role in Syt binding. The structures suggest that the BoNT/DC ganglioside binding sites are independent of the protein receptor binding site. Our results reveal the remarkable versatility in the receptor recognition of the BoNTs.
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Crystal Structures of Botulinum Neurotoxin DC in Complex with Its Protein Receptors Synaptotagmin I and II.,Berntsson RP, Peng L, Svensson LM, Dong M, Stenmark P Structure. 2013 Sep 3;21(9):1602-11. doi: 10.1016/j.str.2013.06.026. Epub 2013, Aug 8. PMID:23932591<ref>PMID:23932591</ref>
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==See Also==
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*[[Botulinum neurotoxin 3D structures|Botulinum neurotoxin 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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*[[Synaptotagmin 3D structures|Synaptotagmin 3D structures]]
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</div>
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<div class="pdbe-citations 4isr" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bacillus botulinus van ermengem 1896]]
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[[Category: Clostridium botulinum]]
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[[Category: Berntsson, R P.A]]
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[[Category: Large Structures]]
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[[Category: Dong, M]]
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[[Category: Rattus norvegicus]]
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[[Category: Peng, L]]
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[[Category: Berntsson RP-A]]
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[[Category: Stenmark, P]]
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[[Category: Dong M]]
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[[Category: Svensson, L M]]
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[[Category: Peng L]]
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[[Category: Membrane binding]]
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[[Category: Stenmark P]]
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[[Category: Synaptotagmin and ganglioside binding]]
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[[Category: Svensson LM]]
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[[Category: Toxin]]
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Current revision

Binding domain of Botulinum neurotoxin DC in complex with rat synaptotagmin II

PDB ID 4isr

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