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3rtl

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==Staphylococcus aureus heme-bound IsdB-N2==
==Staphylococcus aureus heme-bound IsdB-N2==
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<StructureSection load='3rtl' size='340' side='right' caption='[[3rtl]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
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<StructureSection load='3rtl' size='340' side='right'caption='[[3rtl]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3rtl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Staan Staan]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RTL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RTL FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3rtl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_N315 Staphylococcus aureus subsp. aureus N315]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RTL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RTL FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.453&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">frpB, isdB, SA0976, sasJ, sirH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158879 STAAN])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rtl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rtl OCA], [http://pdbe.org/3rtl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3rtl RCSB], [http://www.ebi.ac.uk/pdbsum/3rtl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3rtl ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rtl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rtl OCA], [https://pdbe.org/3rtl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rtl RCSB], [https://www.ebi.ac.uk/pdbsum/3rtl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rtl ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/ISDB_STAAN ISDB_STAAN]] Seems to function as the primary receptor for hemoglobin since its inactivation inhibits the ability of S.aureus to bind hemoglobin. Binds hemoglobin in a dose-dependent way. Required for S.aureus growth using hemoglobin as the sole iron source. Also required for virulence. IsdA and/or IsdB promote resistance to hydrogen peroxide and killing by neutrophils (By similarity).
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[https://www.uniprot.org/uniprot/ISDB_STAAN ISDB_STAAN] Seems to function as the primary receptor for hemoglobin since its inactivation inhibits the ability of S.aureus to bind hemoglobin. Binds hemoglobin in a dose-dependent way. Required for S.aureus growth using hemoglobin as the sole iron source. Also required for virulence. IsdA and/or IsdB promote resistance to hydrogen peroxide and killing by neutrophils (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Iron is an essential requirement for life for nearly all organisms. The human pathogen Staphylococcus aureus is able to acquire iron from the heme cofactor of hemoglobin (Hb) released from lysed erythrocytes. IsdB, the predominant Hb receptor of S. aureus, is a cell wall-anchored protein that is composed of two NEAT domains. The N-terminal NEAT domain (IsdB-N1) binds Hb, and the C-terminal NEAT domain (IsdB-N2) relays heme to IsdA for transport into the cell. Here we present the 1.45 A resolution X-ray crystal structure of the IsdB-N2-heme complex. While the structure largely conforms to the eight-strand beta-sandwich fold seen in other NEAT domains such as IsdA-N and uses a conserved Tyr residue to coordinate heme-iron, a Met residue is also involved in iron coordination, resulting in a novel Tyr-Met hexacoordinate heme-iron state. The kinetics of the transfer of heme from IsdB-N2 to IsdA-N can be modeled as a two-step process. The rate of transfer of heme between the isolated NEAT domains (82 s(-1)) was found to be similar to that measured for the full-length proteins. Replacing the iron coordinating Met with Leu did not abrogate high-affinity heme binding but did reduce the heme transfer rate constant by more than half. This unusual Met-Tyr heme coordination may also bestow properties on IsdB that help it to bind heme in different oxidation states or extract heme from hemoglobin.
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Unique Heme-Iron Coordination by the Hemoglobin Receptor IsdB of Staphylococcus aureus.,Gaudin CF, Grigg JC, Arrieta AL, Murphy ME Biochemistry. 2011 Jun 21;50(24):5443-5452. Epub 2011 May 26. PMID:21574663<ref>PMID:21574663</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3rtl" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Staan]]
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[[Category: Large Structures]]
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[[Category: Arrieta, A L]]
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[[Category: Staphylococcus aureus subsp. aureus N315]]
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[[Category: Gaudin, C F.M]]
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[[Category: Arrieta AL]]
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[[Category: Grigg, J C]]
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[[Category: Gaudin CFM]]
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[[Category: Murphy, M E.P]]
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[[Category: Grigg JC]]
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[[Category: Cell wall]]
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[[Category: Murphy MEP]]
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[[Category: Heme binding]]
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[[Category: Heme protein]]
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[[Category: Heme uptake]]
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[[Category: Metal transport]]
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[[Category: Neat domain]]
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Current revision

Staphylococcus aureus heme-bound IsdB-N2

PDB ID 3rtl

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