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| | ==Crystal structure of CTX A2 from Taiwan Cobra (Naja naja atra)== | | ==Crystal structure of CTX A2 from Taiwan Cobra (Naja naja atra)== |
| - | <StructureSection load='4om4' size='340' side='right' caption='[[4om4]], [[Resolution|resolution]] 2.74Å' scene=''> | + | <StructureSection load='4om4' size='340' side='right'caption='[[4om4]], [[Resolution|resolution]] 2.74Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4om4]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OM4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OM4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4om4]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OM4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OM4 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4om5|4om5]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.74Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4om4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4om4 OCA], [http://pdbe.org/4om4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4om4 RCSB], [http://www.ebi.ac.uk/pdbsum/4om4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4om4 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4om4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4om4 OCA], [https://pdbe.org/4om4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4om4 RCSB], [https://www.ebi.ac.uk/pdbsum/4om4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4om4 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CTXA2_NAJAT CTXA2_NAJAT]] Basic protein that bind to cell membrane and depolarizes cardiomyocytes. It also shows lytic activities, but 2-fold less important than that of CTX-A4. It binds to the integrin alpha-V/beta-3 with a moderate affinity. It may interact with sulfatides in the cell membrane which induces pore formation and cell internalization and is responsible for cytotoxicity in cardiomyocytes. It also may target the mitochondrial membrane and induces mitochondrial swelling and fragmentation.<ref>PMID:9398182</ref> | + | [https://www.uniprot.org/uniprot/3SA2_NAJAT 3SA2_NAJAT] Basic protein that binds to cell membrane and depolarizes cardiomyocytes. It also shows lytic activities, but 2-fold less important than that of CTX-A4. It binds to the integrin alpha-V/beta-3 (ITGAV/ITGB3) with a moderate affinity. It may interact with sulfatides in the cell membrane which induces pore formation and cell internalization and is responsible for cytotoxicity in cardiomyocytes. It also may target the mitochondrial membrane and induce mitochondrial swelling and fragmentation.<ref>PMID:9398182</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Cardiotoxin|Cardiotoxin]] | + | *[[Cardiotoxin 3D structures|Cardiotoxin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Naja atra]] | | [[Category: Naja atra]] |
| - | [[Category: Chang, C I]] | + | [[Category: Chang CI]] |
| - | [[Category: Lin, C C]] | + | [[Category: Lin CC]] |
| - | [[Category: Wu, W G]] | + | [[Category: Wu WG]] |
| - | [[Category: Endocytosis]]
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| - | [[Category: Five beta sheet]]
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| - | [[Category: Heparan sulfate]]
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| - | [[Category: Heparin]]
| + | |
| - | [[Category: Three functional loop]]
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| - | [[Category: Toxin]]
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| Structural highlights
Function
3SA2_NAJAT Basic protein that binds to cell membrane and depolarizes cardiomyocytes. It also shows lytic activities, but 2-fold less important than that of CTX-A4. It binds to the integrin alpha-V/beta-3 (ITGAV/ITGB3) with a moderate affinity. It may interact with sulfatides in the cell membrane which induces pore formation and cell internalization and is responsible for cytotoxicity in cardiomyocytes. It also may target the mitochondrial membrane and induce mitochondrial swelling and fragmentation.[1]
Publication Abstract from PubMed
Cobra cardiotoxins (CTXs) are a family of three-fingered basic polypeptides known to interact with diverse targets such as heparan sulfates, sulfatides and integrins on cell surfaces. After CTXs bind to the membrane surface, they are internalized to intracellular space and exert their cytotoxicity with an unknown mechanism. By the combined in vitro kinetic binding, 3D X-ray structure determination, and cell biology studies on the naturally abundant CTX homologues from the Taiwanese cobra, we showed that slight variations on the spatial distribution of positively charged or hydrophobic domains among CTX A2, A3 and A4 could lead to significant changes in their endocytotic pathways and action mechanisms via distinct sulfated glycoconjugates mediated processes. The intracellular locations of these structurally similar CTXs after internalization are shown to vary between the mitochondria and lysosomes via either dynamin2-dependent or -independent processes with distinct membrane cholesterol sensitivity. Evidence is presented to suggest that the shifting between the sulfated glycoconjugates as distinct targets of CTX A2, A3 and A4 might play roles in the co-evolutionary arms race between venomous snake toxins to cope with different membrane repair mechanisms at the cellular levels. The sensitivity of endocytotic routes to the spatial distribution of positively charged or hydrophobic domains may provide an explanation for the diverse endocytosis pathways of other cell-penetrating basic polypeptides.
Endocytotic Routes of Cobra Cardiotoxins Depend on the Spatial Distribution of Positively Charged and Hydrophobic Domains to Target Distinct Types of Sulfated Glycoconjugates on Cell Surface.,Lee SC, Lin CC, Wang CH, Wu PL, Huang HW, Chang CI, Wu WG J Biol Chem. 2014 Jun 4. pii: jbc.M114.557157. PMID:24898246[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Jang JY, Krishnaswamy T, Kumar S, Jayaraman G, Yang PW, Yu C. Comparison of the hemolytic activity and solution structures of two snake venom cardiotoxin analogues which only differ in their N-terminal amino acid. Biochemistry. 1997 Dec 2;36(48):14635-41. PMID:9398182 doi:10.1021/bi971107a
- ↑ Lee SC, Lin CC, Wang CH, Wu PL, Huang HW, Chang CI, Wu WG. Endocytotic Routes of Cobra Cardiotoxins Depend on the Spatial Distribution of Positively Charged and Hydrophobic Domains to Target Distinct Types of Sulfated Glycoconjugates on Cell Surface. J Biol Chem. 2014 Jun 4. pii: jbc.M114.557157. PMID:24898246 doi:http://dx.doi.org/10.1074/jbc.M114.557157
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