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3spw

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==Structure of Osh4p/Kes1p in complex with phosphatidylinositol 4-phosphate==
==Structure of Osh4p/Kes1p in complex with phosphatidylinositol 4-phosphate==
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<StructureSection load='3spw' size='340' side='right' caption='[[3spw]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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<StructureSection load='3spw' size='340' side='right'caption='[[3spw]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3spw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SPW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SPW FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3spw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SPW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SPW FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=T7M:(2R)-1-(HEPTADECANOYLOXY)-3-{[(R)-HYDROXY{[(1R,2R,3R,4R,5S,6R)-2,3,5,6-TETRAHYDROXY-4-(PHOSPHONOOXY)CYCLOHEXYL]OXY}PHOSPHORYL]OXY}PROPAN-2-YL+(5Z,8Z,11Z,14Z)-ICOSA-5,8,11,14-TETRAENOATE'>T7M</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1zhz|1zhz]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=T7M:(2R)-1-(HEPTADECANOYLOXY)-3-{[(R)-HYDROXY{[(1R,2R,3R,4R,5S,6R)-2,3,5,6-TETRAHYDROXY-4-(PHOSPHONOOXY)CYCLOHEXYL]OXY}PHOSPHORYL]OXY}PROPAN-2-YL+(5Z,8Z,11Z,14Z)-ICOSA-5,8,11,14-TETRAENOATE'>T7M</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KES1, LPI3C, OSH4, P2614, YPL145C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3spw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3spw OCA], [https://pdbe.org/3spw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3spw RCSB], [https://www.ebi.ac.uk/pdbsum/3spw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3spw ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3spw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3spw OCA], [http://pdbe.org/3spw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3spw RCSB], [http://www.ebi.ac.uk/pdbsum/3spw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3spw ProSAT]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/KES1_YEAST KES1_YEAST]] Plays a role in ergosterol synthesis.
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[https://www.uniprot.org/uniprot/KES1_YEAST KES1_YEAST] Plays a role in ergosterol synthesis.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Osh/Orp proteins transport sterols between organelles and are involved in phosphoinositide metabolism. The link between these two aspects remains elusive. Using novel assays, we address the influence of membrane composition on the ability of Osh4p/Kes1p to extract, deliver, or transport dehydroergosterol (DHE). Surprisingly, phosphatidylinositol 4-phosphate (PI(4)P) specifically inhibited DHE extraction because PI(4)P was itself efficiently extracted by Osh4p. We solve the structure of the Osh4p-PI(4)P complex and reveal how Osh4p selectively substitutes PI(4)P for sterol. Last, we show that Osh4p quickly exchanges DHE for PI(4)P and, thereby, can transport these two lipids between membranes along opposite routes. These results suggest a model in which Osh4p transports sterol from the ER to late compartments pinpointed by PI(4)P and, in turn, transports PI(4)P backward. Coupled to PI(4)P metabolism, this transport cycle would create sterol gradients. Because the residues that recognize PI(4)P are conserved in Osh4p homologues, other Osh/Orp are potential sterol/phosphoinositol phosphate exchangers.
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Osh4p exchanges sterols for phosphatidylinositol 4-phosphate between lipid bilayers.,de Saint-Jean M, Delfosse V, Douguet D, Chicanne G, Payrastre B, Bourguet W, Antonny B, Drin G J Cell Biol. 2011 Dec 12;195(6):965-78. PMID:22162133<ref>PMID:22162133</ref>
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==See Also==
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*[[Oxysterol-binding protein homolog|Oxysterol-binding protein homolog]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3spw" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Baker's yeast]]
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[[Category: Large Structures]]
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[[Category: Antonny, B]]
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[[Category: Saccharomyces cerevisiae S288C]]
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[[Category: Bourguet, W]]
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[[Category: Antonny B]]
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[[Category: Delfosse, V]]
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[[Category: Bourguet W]]
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[[Category: Douguet, D]]
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[[Category: Delfosse V]]
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[[Category: Drin, G]]
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[[Category: Douguet D]]
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[[Category: Saint-Jean, M de]]
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[[Category: Drin G]]
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[[Category: Lipid binding protein]]
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[[Category: De Saint-Jean M]]
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[[Category: Protein binding]]
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Current revision

Structure of Osh4p/Kes1p in complex with phosphatidylinositol 4-phosphate

PDB ID 3spw

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