3q37

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==Identification of Amino Acids that Account for Long-Range Interactions in Proteins Using Two Triosephosphate Isomerases from Pathogenic Trypanosomes.==
==Identification of Amino Acids that Account for Long-Range Interactions in Proteins Using Two Triosephosphate Isomerases from Pathogenic Trypanosomes.==
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<StructureSection load='3q37' size='340' side='right' caption='[[3q37]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
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<StructureSection load='3q37' size='340' side='right'caption='[[3q37]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3q37]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Trycr Trycr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q37 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3Q37 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3q37]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei Trypanosoma brucei brucei] and [https://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q37 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q37 FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1tcd|1tcd]], [[5tim|5tim]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TIM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5693 TRYCR])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q37 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q37 OCA], [https://pdbe.org/3q37 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q37 RCSB], [https://www.ebi.ac.uk/pdbsum/3q37 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q37 ProSAT]</span></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3q37 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q37 OCA], [http://pdbe.org/3q37 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3q37 RCSB], [http://www.ebi.ac.uk/pdbsum/3q37 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3q37 ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/TPIS_TRYCR TPIS_TRYCR] [https://www.uniprot.org/uniprot/TPIS_TRYBB TPIS_TRYBB]
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For a better comprehension of the structure-function relationship in proteins it is necessary to identify the amino acids that are relevant for measurable protein functions. Because of the numerous contacts that amino acids establish within proteins and the cooperative nature of their interactions, it is difficult to achieve this goal. Thus, the study of protein-ligand interactions is usually focused on local environmental structural differences. Here, using a pair of triosephosphate isomerase enzymes with extremely high homology from two different organisms, we demonstrate that the control of a seventy-fold difference in reactivity of the interface cysteine is located in several amino acids from two structurally unrelated regions that do not contact the cysteine sensitive to the sulfhydryl reagent methylmethane sulfonate, nor the residues in its immediate vicinity. The change in reactivity is due to an increase in the apparent pKa of the interface cysteine produced by the mutated residues. Our work, which involved grafting systematically portions of one protein into the other protein, revealed unsuspected and multisite long-range interactions that modulate the properties of the interface cysteines and has general implications for future studies on protein structure-function relationships.
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Identification of amino acids that account for long-range interactions in two triosephosphate isomerases from pathogenic trypanosomes.,Garcia-Torres I, Cabrera N, Torres-Larios A, Rodriguez-Bolanos M, Diaz-Mazariegos S, Gomez-Puyou A, Perez-Montfort R PLoS One. 2011 Apr 18;6(4):e18791. PMID:21533154<ref>PMID:21533154</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3q37" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[Triose Phosphate Isomerase|Triose Phosphate Isomerase]]
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*[[Triose phosphate isomerase 3D structures|Triose phosphate isomerase 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Triose-phosphate isomerase]]
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[[Category: Large Structures]]
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[[Category: Trycr]]
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[[Category: Trypanosoma brucei brucei]]
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[[Category: Cabrera, N]]
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[[Category: Trypanosoma cruzi]]
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[[Category: Garcia-Torres, I]]
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[[Category: Cabrera N]]
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[[Category: Gomez-Puyou, A]]
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[[Category: Garcia-Torres I]]
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[[Category: Perez-Montfort, R]]
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[[Category: Gomez-Puyou A]]
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[[Category: Rodriguez-Bolanos, M]]
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[[Category: Perez-Montfort R]]
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[[Category: Torres-Larios, A]]
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[[Category: Rodriguez-Bolanos M]]
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[[Category: Isomerase]]
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[[Category: Torres-Larios A]]
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[[Category: Tim barrel]]
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Current revision

Identification of Amino Acids that Account for Long-Range Interactions in Proteins Using Two Triosephosphate Isomerases from Pathogenic Trypanosomes.

PDB ID 3q37

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