1l5a

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[[Image:1l5a.gif|left|200px]]
 
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{{Structure
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==Crystal Structure of VibH, an NRPS Condensation Enzyme==
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|PDB= 1l5a |SIZE=350|CAPTION= <scene name='initialview01'>1l5a</scene>, resolution 2.55&Aring;
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<StructureSection load='1l5a' size='340' side='right'caption='[[1l5a]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1l5a]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L5A FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55&#8491;</td></tr>
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|GENE= vibH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l5a OCA], [https://pdbe.org/1l5a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l5a RCSB], [https://www.ebi.ac.uk/pdbsum/1l5a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l5a ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1l5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l5a OCA], [http://www.ebi.ac.uk/pdbsum/1l5a PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1l5a RCSB]</span>
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[https://www.uniprot.org/uniprot/Q9KTV9_VIBCH Q9KTV9_VIBCH]
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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'''Crystal Structure of VibH, an NRPS Condensation Enzyme'''
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l5/1l5a_consurf.spt"</scriptWhenChecked>
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==Overview==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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Nonribosomal peptide synthetases (NRPSs) are large, multidomain enzymes that biosynthesize medically important natural products. We report the crystal structure of the free-standing NRPS condensation (C) domain VibH, which catalyzes amide bond formation in the synthesis of vibriobactin, a Vibrio cholerae siderophore. Despite low sequence identity, NRPS condensation enzymes are structurally related to chloramphenicol acetyltransferase (CAT) and dihydrolipoamide acyltransferases. However, although the latter enzymes are homotrimers, VibH is a monomeric pseudodimer. The VibH structure is representative of both NRPS condensation and epimerization domains, as well as the condensation-variant cyclization domains, which are all expected to be monomers. Surprisingly, despite favorable positioning in the active site, a universally conserved histidine important in CAT and in other C domains is not critical for general base catalysis in VibH.
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==About this Structure==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l5a ConSurf].
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1L5A is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5A OCA].
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<div style="clear:both"></div>
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__TOC__
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==Reference==
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</StructureSection>
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The structure of VibH represents nonribosomal peptide synthetase condensation, cyclization and epimerization domains., Keating TA, Marshall CG, Walsh CT, Keating AE, Nat Struct Biol. 2002 Jul;9(7):522-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12055621 12055621]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Vibrio cholerae]]
[[Category: Vibrio cholerae]]
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[[Category: Keating, A E.]]
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[[Category: Keating AE]]
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[[Category: Keating, T A.]]
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[[Category: Keating TA]]
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[[Category: Marshall, C G.]]
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[[Category: Marshall CG]]
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[[Category: Walsh, C T.]]
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[[Category: Walsh CT]]
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[[Category: amide synthase]]
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[[Category: nonribosomal peptide synthetase]]
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[[Category: nrps condensation domain]]
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[[Category: vibriobactin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:58:13 2008''
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Current revision

Crystal Structure of VibH, an NRPS Condensation Enzyme

PDB ID 1l5a

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