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4h1w

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==E1 structure of the (SR) Ca2+-ATPase in complex with Sarcolipin==
==E1 structure of the (SR) Ca2+-ATPase in complex with Sarcolipin==
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<StructureSection load='4h1w' size='340' side='right' caption='[[4h1w]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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<StructureSection load='4h1w' size='340' side='right'caption='[[4h1w]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4h1w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H1W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H1W FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4h1w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H1W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H1W FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2c8k|2c8k]], [[1t5s|1t5s]], [[1t5t|1t5t]], [[3b9b|3b9b]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Calcium-transporting_ATPase Calcium-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.8 3.6.3.8] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h1w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h1w OCA], [https://pdbe.org/4h1w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h1w RCSB], [https://www.ebi.ac.uk/pdbsum/4h1w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h1w ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h1w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h1w OCA], [http://pdbe.org/4h1w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4h1w RCSB], [http://www.ebi.ac.uk/pdbsum/4h1w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4h1w ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/AT2A1_RABIT AT2A1_RABIT] This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction (By similarity).
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The contraction and relaxation of muscle cells is controlled by the successive rise and fall of cytosolic Ca(2+), initiated by the release of Ca(2+) from the sarcoplasmic reticulum and terminated by re-sequestration of Ca(2+) into the sarcoplasmic reticulum as the main mechanism of Ca(2+) removal. Re-sequestration requires active transport and is catalysed by the sarcoplasmic reticulum Ca(2+)-ATPase (SERCA), which has a key role in defining the contractile properties of skeletal and heart muscle tissue. The activity of SERCA is regulated by two small, homologous membrane proteins called phospholamban (PLB, also known as PLN) and sarcolipin (SLN). Detailed structural information explaining this regulatory mechanism has been lacking, and the structural features defining the pathway through which cytoplasmic Ca(2+) enters the intramembranous binding sites of SERCA have remained unknown. Here we report the crystal structure of rabbit SERCA1a (also known as ATP2A1) in complex with SLN at 3.1 A resolution. The regulatory SLN traps the Ca(2+)-ATPase in a previously undescribed E1 state, with exposure of the Ca(2+) sites through an open cytoplasmic pathway stabilized by Mg(2+). The structure suggests a mechanism for selective Ca(2+) loading and activation of SERCA, and provides new insight into how SLN and PLB inhibition arises from stabilization of this E1 intermediate state without bound Ca(2+). These findings may prove useful in studying how autoinhibitory domains of other ion pumps modulate transport across biological membranes.
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The sarcolipin-bound calcium pump stabilizes calcium sites exposed to the cytoplasm.,Winther AM, Bublitz M, Karlsen JL, Moller JV, Hansen JB, Nissen P, Buch-Pedersen MJ Nature. 2013 Mar 14;495(7440):265-9. doi: 10.1038/nature11900. Epub 2013 Mar 3. PMID:23455424<ref>PMID:23455424</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4h1w" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[ATPase|ATPase]]
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*[[ATPase 3D structures|ATPase 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Calcium-transporting ATPase]]
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[[Category: Large Structures]]
[[Category: Oryctolagus cuniculus]]
[[Category: Oryctolagus cuniculus]]
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[[Category: Bublitz, M]]
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[[Category: Bublitz M]]
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[[Category: Buch-Pedersen, M J]]
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[[Category: Buch-Pedersen MJ]]
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[[Category: Hansen, J B]]
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[[Category: Hansen JB]]
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[[Category: Karlsen, J]]
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[[Category: Karlsen J]]
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[[Category: Moller, J V]]
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[[Category: Moller JV]]
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[[Category: Nissen, P]]
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[[Category: Nissen P]]
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[[Category: Winther, A M.L]]
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[[Category: Winther A-ML]]
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[[Category: Ca2+ transport]]
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[[Category: Hydrolase-hydrolase regulator complex]]
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[[Category: Membrane protein]]
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[[Category: P-type atpase]]
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[[Category: Phospholamban]]
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[[Category: Sarcolipin]]
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Current revision

E1 structure of the (SR) Ca2+-ATPase in complex with Sarcolipin

PDB ID 4h1w

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