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| - | [[Image:1l6l.gif|left|200px]]
| + | <span style='background-color: yellow;'>For additional information see '''2009, December:''' at [[Retractions and Fraud]].</span></br>REMOVED: The PDB entry 1l6l was removed. |
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| - | {{Structure
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| - | |PDB= 1l6l |SIZE=350|CAPTION= <scene name='initialview01'>1l6l</scene>, resolution 2.3Å
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| - | |SITE=
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| - | |LIGAND= <scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>
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| - | |ACTIVITY=
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| - | |GENE=
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| - | |DOMAIN=
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| - | |RELATEDENTRY=[[1l6k|1L6K]]
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| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1l6l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l6l OCA], [http://www.ebi.ac.uk/pdbsum/1l6l PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1l6l RCSB]</span>
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| - | }}
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| - | '''Structures of Apolipoprotein A-II and a Lipid Surrogate Complex Provide Insights into Apolipoprotein-Lipid Interactions''' | + | |
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| - | ==Overview==
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| - | Apolipoproteins A-I and A-II form the major protein constituents of high-density lipid particles (HDL), the concentration of which is inversely correlated with the frequency of heart disease in humans. Although the physiological role of apolipoprotein A-II is unclear, evidence for its involvement in free fatty acid metabolism in mice has recently been obtained. Currently, the best characterized activity of apolipoprotein A-II is its potent antagonism of the anti-atherogenic and anti-inflammatory activities of apolipoprotein A-I, probably due to its competition with the latter for lipid acyl side chains in HDL. Many interactions of apolipoprotein A-I with enzymes and proteins involved in reverse cholesterol transport and HDL maturation are mediated by lipid-bound protein. The structural bases of interaction with lipids are expected to be common to exchangeable apolipoproteins and attributable to amphipathic alpha-helices present in each of them. Thus, characterization of apolipoprotein-lipid interactions in any apolipoprotein is likely to provide information that is applicable to the entire class. We report structures of human apolipoprotein A-II and its complex with beta-octyl glucoside, a widely used lipid surrogate. The former shows that disulfide-linked dimers of apolipoprotein A-II form amphipathic alpha-helices which aggregate into tetramers. Dramatic changes, observed in the presence of beta-octyl glucoside, might provide clues to the structural basis for its antagonism of apolipoprotein A-I. Additionally, excursions of individual molecules of apolipoprotein A-II from a common helical architecture in both structures indicate that lipid-bound apolipoproteins are likely to have an ensemble of related conformations. These structures provide the first experimental paradigm for description of apolipoprotein-lipid interactions at the atomic level.
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| - | ==About this Structure==
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| - | 1L6L is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L6L OCA].
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| - | ==Reference==
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| - | Structures of apolipoprotein A-II and a lipid-surrogate complex provide insights into apolipoprotein-lipid interactions., Kumar MS, Carson M, Hussain MM, Murthy HM, Biochemistry. 2002 Oct 1;41(39):11681-91. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12269810 12269810]
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| - | [[Category: Homo sapiens]]
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| - | [[Category: Single protein]]
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| - | [[Category: Carson, M.]]
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| - | [[Category: Hussain, M M.]]
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| - | [[Category: Kumar, M S.]]
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| - | [[Category: Murthy, H M.K.]]
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| - | [[Category: apolipoprotein]]
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| - | [[Category: apolipoprotein a-ii]]
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| - | [[Category: cholesterol metabolism]]
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| - | [[Category: helix]]
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| - | [[Category: high density lipid]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:58:49 2008''
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