3s2u

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==Crystal structure of the Pseudomonas aeruginosa MurG:UDP-GlcNAc substrate complex==
==Crystal structure of the Pseudomonas aeruginosa MurG:UDP-GlcNAc substrate complex==
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<StructureSection load='3s2u' size='340' side='right' caption='[[3s2u]], [[Resolution|resolution]] 2.23&Aring;' scene=''>
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<StructureSection load='3s2u' size='340' side='right'caption='[[3s2u]], [[Resolution|resolution]] 2.23&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3s2u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S2U FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3s2u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S2U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S2U FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.23&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">murG, PA4412 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Undecaprenyldiphospho-muramoylpentapeptide_beta-N-_acetylglucosaminyltransferase Undecaprenyldiphospho-muramoylpentapeptide beta-N- acetylglucosaminyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.227 2.4.1.227] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s2u OCA], [https://pdbe.org/3s2u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s2u RCSB], [https://www.ebi.ac.uk/pdbsum/3s2u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s2u ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s2u OCA], [http://pdbe.org/3s2u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3s2u RCSB], [http://www.ebi.ac.uk/pdbsum/3s2u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3s2u ProSAT]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/MURG_PSEAE MURG_PSEAE]] Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc-(pentapeptide)GlcNAc (lipid intermediate II) (By similarity).
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[https://www.uniprot.org/uniprot/MURG_PSEAE MURG_PSEAE] Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc-(pentapeptide)GlcNAc (lipid intermediate II) (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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MurG is an essential bacterial glycosyltransferase enzyme in Pseudomonas aeruginosa performing one of the key membrane steps of peptidoglycan synthesis catalyzing the transfer of N-acetyl glucosamine (GlcNAc) from its donor substrate, UDP-GlcNAc, to the acceptor substrate Lipid I. We have solved the crystal structure of the complex between Pseudomonas aeruginosa MurG and UDP-GlcNAc and compared it with the previously solved complex from E. coli. The structure reveals a large-scale conformational change in the relative orientations of the N- and C-terminal domains, which has the effect of widening the cofactor binding site and displacing the UDP-GlcNAc donor. These results suggest new opportunities to design potent inhibitors of peptidoglycan biosynthesis.
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Crystal structure of the Pseudomonas aeruginosa MurG:UDP-GlcNAc substrate complex.,Brown K, Vial SC, Dedi N, Westcott J, Scally S, Bugg TD, Charlton PA, Cheetham GM Protein Pept Lett. 2012 Sep 4. PMID:22973843<ref>PMID:22973843</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3s2u" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[O-GlcNAc transferase|O-GlcNAc transferase]]
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*[[O-GlcNAc transferase 3D structures|O-GlcNAc transferase 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Pseae]]
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[[Category: Large Structures]]
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[[Category: Undecaprenyldiphospho-muramoylpentapeptide beta-N- acetylglucosaminyltransferase]]
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[[Category: Pseudomonas aeruginosa PAO1]]
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[[Category: Brown, K]]
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[[Category: Brown K]]
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[[Category: Bugg, T D.H]]
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[[Category: Bugg TDH]]
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[[Category: Charlton, P A]]
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[[Category: Charlton PA]]
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[[Category: Cheetham, G M.T]]
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[[Category: Cheetham GMT]]
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[[Category: Dedi, N]]
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[[Category: Dedi N]]
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[[Category: Scally, S]]
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[[Category: Scally S]]
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[[Category: Vial, S C.M]]
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[[Category: Vial SCM]]
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[[Category: Westcott, J]]
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[[Category: Westcott J]]
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[[Category: N-acetylglucosaminyl transferase]]
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[[Category: Transferase]]
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Current revision

Crystal structure of the Pseudomonas aeruginosa MurG:UDP-GlcNAc substrate complex

PDB ID 3s2u

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