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| | ==Structural insigths into dsRNA binding and RNA silencing suppression by NS3 protein of rice hoja blanca tenuivirus== | | ==Structural insigths into dsRNA binding and RNA silencing suppression by NS3 protein of rice hoja blanca tenuivirus== |
| - | <StructureSection load='3ajf' size='340' side='right' caption='[[3ajf]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='3ajf' size='340' side='right'caption='[[3ajf]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3ajf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhbv Rhbv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AJF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AJF FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3ajf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rice_hoja_blanca_virus_-_cr Rice hoja blanca virus - cr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AJF FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NS3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=480611 RHBV])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ajf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ajf OCA], [https://pdbe.org/3ajf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ajf RCSB], [https://www.ebi.ac.uk/pdbsum/3ajf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ajf ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ajf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ajf OCA], [http://pdbe.org/3ajf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ajf RCSB], [http://www.ebi.ac.uk/pdbsum/3ajf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ajf ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/VSR_RHBVC VSR_RHBVC]] Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), presumably through the binding of dsRNA (By similarity). | + | [https://www.uniprot.org/uniprot/VSR_RHBVC VSR_RHBVC] Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), presumably through the binding of dsRNA (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Rhbv]] | + | [[Category: Large Structures]] |
| - | [[Category: Yuan, Y A]] | + | [[Category: Rice hoja blanca virus - cr]] |
| - | [[Category: Negative strand rna virus]]
| + | [[Category: Yuan YA]] |
| - | [[Category: Rna silencing suppression]] | + | |
| - | [[Category: Viral protein]]
| + | |
| Structural highlights
Function
VSR_RHBVC Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), presumably through the binding of dsRNA (By similarity).
Publication Abstract from PubMed
Rice Hoja Blanca Tenuivirus (RHBV), a negative strand RNA virus, has been identified to infect rice and is widely transmitted by the insect vector. NS3 protein encoded by RHBV RNA3 was reported to be a potent RNAi suppressor to counterdefense RNA silencing in plants, insect cells, and mammalian cells. Here, we report the crystal structure of the N-terminal domain of RHBV NS3 (residues 21-114) at 2.0 A. RHBV NS3 N-terminal domain forms a dimer by two pairs of alpha-helices in an anti-parallel mode, with one surface harboring a shallow groove at the dimension of 20 A x 30 A for putative dsRNA binding. In vitro RNA binding assay and RNA silencing suppression assay have demonstrated that the structural conserved residues located along this shallow groove, such as Arg50, His51, Lys77, and His85, participate in dsRNA binding and RNA silencing suppression. Our results provide the initial structural implications in understanding the RNAi suppression mechanism by RHBV NS3.
Structural implications into dsRNA binding and RNA silencing suppression by NS3 protein of Rice Hoja Blanca Tenuivirus.,Yang X, Tan SH, Teh YJ, Yuan YA RNA. 2011 May;17(5):903-11. Epub 2011 Apr 1. PMID:21460234[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Yang X, Tan SH, Teh YJ, Yuan YA. Structural implications into dsRNA binding and RNA silencing suppression by NS3 protein of Rice Hoja Blanca Tenuivirus. RNA. 2011 May;17(5):903-11. Epub 2011 Apr 1. PMID:21460234 doi:10.1261/rna.2552811
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