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| | ==The Crystal Structure of Immunity Factor for SPN (IFS)== | | ==The Crystal Structure of Immunity Factor for SPN (IFS)== |
| - | <StructureSection load='3qb2' size='340' side='right' caption='[[3qb2]], [[Resolution|resolution]] 2.50Å' scene=''> | + | <StructureSection load='3qb2' size='340' side='right'caption='[[3qb2]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3qb2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_scarlatinae"_klein_1884 "micrococcus scarlatinae" klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QB2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QB2 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3qb2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QB2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QB2 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3pnt|3pnt]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qb2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qb2 OCA], [https://pdbe.org/3qb2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qb2 RCSB], [https://www.ebi.ac.uk/pdbsum/3qb2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qb2 ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ifs ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1314 "Micrococcus scarlatinae" Klein 1884])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qb2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qb2 OCA], [http://pdbe.org/3qb2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3qb2 RCSB], [http://www.ebi.ac.uk/pdbsum/3qb2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3qb2 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q2VJ58_STRPY Q2VJ58_STRPY] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Micrococcus scarlatinae klein 1884]] | + | [[Category: Large Structures]] |
| - | [[Category: Ellenberger, T]] | + | [[Category: Streptococcus pyogenes]] |
| - | [[Category: Smith, C L]] | + | [[Category: Ellenberger T]] |
| - | [[Category: Glycohydrolase inhibitor]] | + | [[Category: Smith CL]] |
| - | [[Category: Hydrolase inhibitor]]
| + | |
| - | [[Category: Streptococcus pyogenes glycohydrolase toxin]]
| + | |
| Structural highlights
Function
Q2VJ58_STRPY
Publication Abstract from PubMed
The virulence of Gram-positive bacteria is enhanced by toxins like the Streptococcus pyogenes beta-NAD(+) glycohydrolase known as SPN. SPN-producing strains of S. pyogenes additionally express the protein immunity factor for SPN (IFS), which forms an inhibitory complex with SPN. We have determined crystal structures of the SPN-IFS complex and IFS alone, revealing that SPN is structurally related to ADP-ribosyl transferases but lacks the canonical binding site for protein substrates. SPN is instead a highly efficient glycohydrolase with the potential to deplete cellular levels of beta-NAD(+). The protective effect of IFS involves an extensive interaction with the SPN active site that blocks access to beta-NAD(+). The conformation of IFS changes upon binding to SPN, with repacking of an extended C-terminal alpha helix into a compact shape. IFS is an attractive target for the development of novel bacteriocidal compounds functioning by blocking the bacterium's self-immunity to the SPN toxin.
Structural Basis of Streptococcus pyogenes Immunity to Its NAD(+) Glycohydrolase Toxin.,Smith CL, Ghosh J, Elam JS, Pinkner JS, Hultgren SJ, Caparon MG, Ellenberger T Structure. 2011 Feb 9;19(2):192-202. PMID:21300288[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Smith CL, Ghosh J, Elam JS, Pinkner JS, Hultgren SJ, Caparon MG, Ellenberger T. Structural Basis of Streptococcus pyogenes Immunity to Its NAD(+) Glycohydrolase Toxin. Structure. 2011 Feb 9;19(2):192-202. PMID:21300288 doi:10.1016/j.str.2010.12.013
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