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| - | ==CRYSTAL STRUCTURE OF THAUMATIN FROM A AUTO-HARVESTED CRYSTAL, control experiment== | + | ==CRYSTAL STRUCTURE OF THAUMATIN FROM AN AUTO-HARVESTED CRYSTAL, control experiment== |
| - | <StructureSection load='4axu' size='340' side='right' caption='[[4axu]], [[Resolution|resolution]] 1.38Å' scene=''> | + | <StructureSection load='4axu' size='340' side='right'caption='[[4axu]], [[Resolution|resolution]] 1.38Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4axu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thaumatococcus_daniellii Thaumatococcus daniellii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AXU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AXU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4axu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thaumatococcus_daniellii Thaumatococcus daniellii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AXU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AXU FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1kwn|1kwn]], [[1lr2|1lr2]], [[1lr3|1lr3]], [[1lxz|1lxz]], [[1ly0|1ly0]], [[1pp3|1pp3]], [[1rqw|1rqw]], [[1thi|1thi]], [[1thu|1thu]], [[1thv|1thv]], [[1thw|1thw]], [[2a7i|2a7i]], [[2blr|2blr]], [[2blu|2blu]], [[2d8o|2d8o]], [[2d8p|2d8p]], [[2vhk|2vhk]], [[2vhr|2vhr]], [[2vi1|2vi1]], [[2vi2|2vi2]], [[2vi3|2vi3]], [[2vi4|2vi4]], [[2vu6|2vu6]], [[2vu7|2vu7]], [[2wbz|2wbz]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.38Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4axu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4axu OCA], [http://pdbe.org/4axu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4axu RCSB], [http://www.ebi.ac.uk/pdbsum/4axu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4axu ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4axu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4axu OCA], [https://pdbe.org/4axu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4axu RCSB], [https://www.ebi.ac.uk/pdbsum/4axu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4axu ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | == Function == | |
| - | [[http://www.uniprot.org/uniprot/THM1_THADA THM1_THADA]] Taste-modifying protein; intensely sweet-tasting. It is 100000 times sweeter than sucrose on a molar basis. | |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Thaumatococcus daniellii]] | | [[Category: Thaumatococcus daniellii]] |
| - | [[Category: Cipriani, F]] | + | [[Category: Cipriani F]] |
| - | [[Category: Felisaz, F]] | + | [[Category: Felisaz F]] |
| - | [[Category: Landret, C]] | + | [[Category: Landret C]] |
| - | [[Category: Marquez, J A]] | + | [[Category: Marquez JA]] |
| - | [[Category: Rower, M]] | + | [[Category: Rower M]] |
| - | [[Category: Zander, U]] | + | [[Category: Zander U]] |
| - | [[Category: Automation]]
| + | |
| - | [[Category: Crystal mounting]]
| + | |
| - | [[Category: Crystallization microplate]]
| + | |
| - | [[Category: High-throughput crystallization]]
| + | |
| - | [[Category: Plant protein]]
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| Structural highlights
Publication Abstract from PubMed
The use of automated systems for crystallization and X-ray data collection is now widespread. However, these two steps are separated by the need to transfer crystals from crystallization supports to X-ray data-collection supports, which is a difficult manual operation. Here, a new approach is proposed called CrystalDirect (CD) which enables full automation of the crystal-harvesting process. In this approach, crystals are grown on ultrathin films in a newly designed vapour-diffusion crystallization plate and are recovered by excision of the film through laser-induced photoablation. The film pieces containing crystals are then directly attached to a pin for X-ray data collection. This new method eliminates the delicate step of `crystal fishing', thereby enabling full automation of the crystal-mounting process. Additional advantages of this approach include the absence of mechanical stress and that it facilitates handling of microcrystals. The CD crystallization plates are also suitable for in situ crystal screening with minimal X-ray background. This method could enable the operational integration of highly automated crystallization and data-collection facilities, minimizing the delay between crystal identification and diffraction measurements. It can also contribute significantly to the advancement of challenging projects that require the systematic testing of large numbers of crystals.
CrystalDirect: a new method for automated crystal harvesting based on laser-induced photoablation of thin films.,Cipriani F, Rower M, Landret C, Zander U, Felisaz F, Marquez JA Acta Crystallogr D Biol Crystallogr. 2012 Oct;68(Pt 10):1393-9. Epub 2012 Sep 18. PMID:22993093[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Cipriani F, Rower M, Landret C, Zander U, Felisaz F, Marquez JA. CrystalDirect: a new method for automated crystal harvesting based on laser-induced photoablation of thin films. Acta Crystallogr D Biol Crystallogr. 2012 Oct;68(Pt 10):1393-9. Epub 2012 Sep 18. PMID:22993093 doi:http://dx.doi.org/10.1107/S0907444912031459
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