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4luq

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==Crystal structure of virulence effector Tse3 in complex with neutralizer Tsi3==
==Crystal structure of virulence effector Tse3 in complex with neutralizer Tsi3==
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<StructureSection load='4luq' size='340' side='right' caption='[[4luq]], [[Resolution|resolution]] 1.77&Aring;' scene=''>
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<StructureSection load='4luq' size='340' side='right'caption='[[4luq]], [[Resolution|resolution]] 1.77&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4luq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LUQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LUQ FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4luq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LUQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LUQ FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.77&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PA3484 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE]), PA3485 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4luq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4luq OCA], [http://pdbe.org/4luq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4luq RCSB], [http://www.ebi.ac.uk/pdbsum/4luq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4luq ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4luq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4luq OCA], [https://pdbe.org/4luq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4luq RCSB], [https://www.ebi.ac.uk/pdbsum/4luq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4luq ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/TSE3_PSEAE TSE3_PSEAE] Toxin secreted by the H1 type VI (H1-T6SS) secretion system into the periplasm of recipient cells. Degrades peptidoglycan via muramidase activity thereby helping itself to compete with other bacteria (PubMed:21776080). To protect itself, the bacterium synthesizes immunity protein Tsi3 that specifically interacts with and inactivates cognate toxin (PubMed:24025333).<ref>PMID:21776080</ref> <ref>PMID:24025333</ref>
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The warfare among microbial species as well as between pathogens and hosts is fierce, complicated, and continuous. In Pseudomonas aeruginosa, the muramidase effector Tse3 (Type VI secretion exported 3) can be injected into the periplasm of neighboring bacterial competitors by a Type VI secretion apparatus, eventually leading to cell lysis and death. However, P. aeruginosa protects itself from lysis by expressing immune protein Tsi3 (Type six secretion immunity 3). Here, we report the crystal structure of the Tse3-Tsi3 complex at 1.8 A resolution, revealing that Tse3 possesses one open accessible, goose-type lysozyme-like domain with peptidoglycan hydrolysis activity. Calcium ions bind specifically in the Tse3 active site and are identified to be crucial for its bacteriolytic activity. In combination with biochemical studies, the structural basis of self-protection mechanism of Tsi3 is also elucidated, thus providing an understanding and new insights into the effectors of Type VI secretion system.
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Structural Insights on the Bacteriolytic and Self-protection Mechanism of Muramidase Effector Tse3 in Pseudomonas aeruginosa.,Li L, Zhang W, Liu Q, Gao Y, Gao Y, Wang Y, Wang DZ, Li Z, Wang T J Biol Chem. 2013 Oct 18;288(42):30607-13. doi: 10.1074/jbc.C113.506097. Epub, 2013 Sep 11. PMID:24025333<ref>PMID:24025333</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4luq" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Pseae]]
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[[Category: Large Structures]]
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[[Category: Li, L]]
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[[Category: Pseudomonas aeruginosa PAO1]]
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[[Category: Wang, T]]
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[[Category: Li L]]
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[[Category: Zhang, W]]
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[[Category: Wang T]]
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[[Category: Arm]]
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[[Category: Zhang W]]
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[[Category: Catalytic domain]]
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[[Category: Goose type lysozyme]]
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[[Category: Heat like motif]]
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[[Category: Muramidase]]
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[[Category: Protein binding-toxin inhibitor complex]]
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[[Category: Tsi3]]
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Current revision

Crystal structure of virulence effector Tse3 in complex with neutralizer Tsi3

PDB ID 4luq

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