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| | ==Native structure of the UDP-N-acetyl-mannosamine dehydrogenase Cap5O from Staphylococcus aureus== | | ==Native structure of the UDP-N-acetyl-mannosamine dehydrogenase Cap5O from Staphylococcus aureus== |
| - | <StructureSection load='3ojl' size='340' side='right' caption='[[3ojl]], [[Resolution|resolution]] 2.80Å' scene=''> | + | <StructureSection load='3ojl' size='340' side='right'caption='[[3ojl]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3ojl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OJL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OJL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3ojl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OJL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OJL FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ojo|3ojo]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cap5O ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ojl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ojl OCA], [https://pdbe.org/3ojl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ojl RCSB], [https://www.ebi.ac.uk/pdbsum/3ojl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ojl ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ojl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ojl OCA], [http://pdbe.org/3ojl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ojl RCSB], [http://www.ebi.ac.uk/pdbsum/3ojl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ojl ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/P95708_STAAU P95708_STAAU] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Fleurie, A]] | + | [[Category: Large Structures]] |
| - | [[Category: Grangeasse, C]] | + | [[Category: Staphylococcus aureus]] |
| - | [[Category: Gruszczyk, J]] | + | [[Category: Fleurie A]] |
| - | [[Category: Meyer, P]] | + | [[Category: Grangeasse C]] |
| - | [[Category: Morera, S]] | + | [[Category: Gruszczyk J]] |
| - | [[Category: Nessler, S]] | + | [[Category: Meyer P]] |
| - | [[Category: Olivares-Illana, V]] | + | [[Category: Morera S]] |
| - | [[Category: Binary complex]]
| + | [[Category: Nessler S]] |
| - | [[Category: Cofactor nad]]
| + | [[Category: Olivares-Illana V]] |
| - | [[Category: Dimer]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| - | [[Category: Oxidoreduction]]
| + | |
| - | [[Category: Rossmann fold]]
| + | |
| Structural highlights
Function
P95708_STAAU
Publication Abstract from PubMed
Bacterial UDP-sugar dehydrogenases are part of the biosynthesis pathway of extracellular polysaccharides. These compounds act as important virulence factors by protecting the cell from opsonophagocytosis and complement-mediated killing. In Staphylococcus aureus, the protein Cap5O catalyzes the oxidation of UDP-N-acetyl-mannosamine to UDP-N-acetyl-mannosaminuronic acid. Cap5O is crucial for the production of serotype 5 capsular polysaccharide that prevents the interaction of bacteria with both phagocytic and nonphagocytic eukaryotic cells. However, details of its catalytic mechanism remain unknown. We thus crystallized Cap5O and solved the first structure of an UDP-N-acetyl-mannosamine dehydrogenase. This study revealed that the catalytic cysteine makes a disulfide bond that has never been observed in other structurally characterized members of the NDP-sugar dehydrogenase family. Biochemical and mutagenesis experiments demonstrated that the formation of this disulfide bridge regulates the activity of Cap5O. We also identified two arginine residues essential for Cap5O activity. Previous data suggested that Cap5O is activated by tyrosine phosphorylation, so we characterized the phosphorylation site and examined the underlying regulatory mechanism.
Structure analysis of the staphylococcus aureus UDP-N-acetyl-mannosamine dehydrogenase Cap5O involved in capsular polysaccharide biosynthesis.,Gruszczyk J, Fleurie A, Olivares-Illana V, Bechet E, Zanella-Cleon I, Morera S, Meyer P, Pompidor G, Kahn R, Grangeasse C, Nessler S J Biol Chem. 2011 Mar 23. PMID:21454499[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gruszczyk J, Fleurie A, Olivares-Illana V, Bechet E, Zanella-Cleon I, Morera S, Meyer P, Pompidor G, Kahn R, Grangeasse C, Nessler S. Structure analysis of the staphylococcus aureus UDP-N-acetyl-mannosamine dehydrogenase Cap5O involved in capsular polysaccharide biosynthesis. J Biol Chem. 2011 Mar 23. PMID:21454499 doi:10.1074/jbc.M110.216002
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