|
|
| (2 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Structure of Boletus edulis lectin in complex with N,N-diacetyl chitobiose== | | ==Structure of Boletus edulis lectin in complex with N,N-diacetyl chitobiose== |
| - | <StructureSection load='3qdu' size='340' side='right' caption='[[3qdu]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='3qdu' size='340' side='right'caption='[[3qdu]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3qdu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Boletus_edulis Boletus edulis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QDU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QDU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3qdu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Boletus_edulis Boletus edulis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QDU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QDU FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CBS:DI(N-ACETYL-D-GLUCOSAMINE)'>CBS</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3qds|3qds]], [[3qdt|3qdt]], [[3qdv|3qdv]], [[3qdw|3qdw]], [[3qdx|3qdx]], [[3qdy|3qdy]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qdu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qdu OCA], [https://pdbe.org/3qdu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qdu RCSB], [https://www.ebi.ac.uk/pdbsum/3qdu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qdu ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qdu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qdu OCA], [http://pdbe.org/3qdu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3qdu RCSB], [http://www.ebi.ac.uk/pdbsum/3qdu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3qdu ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/BEL_BOLED BEL_BOLED] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 23: |
Line 24: |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Boletus edulis]] | | [[Category: Boletus edulis]] |
| - | [[Category: Bovi, M]] | + | [[Category: Large Structures]] |
| - | [[Category: Capaldi, S]] | + | [[Category: Bovi M]] |
| - | [[Category: Carrizo, M E]] | + | [[Category: Capaldi S]] |
| - | [[Category: Chiarelli, L R]] | + | [[Category: Carrizo ME]] |
| - | [[Category: Galliano, M]] | + | [[Category: Chiarelli LR]] |
| - | [[Category: Monaco, H L]] | + | [[Category: Galliano M]] |
| - | [[Category: Perduca, M]] | + | [[Category: Monaco HL]] |
| - | [[Category: Boletus eduli]]
| + | [[Category: Perduca M]] |
| - | [[Category: Carbohydrate]]
| + | |
| - | [[Category: Lectin]]
| + | |
| - | [[Category: Mushroom]]
| + | |
| - | [[Category: N-diacetyl chitobiose]]
| + | |
| - | [[Category: Sugar binding]]
| + | |
| - | [[Category: Sugar binding protein]]
| + | |
| - | [[Category: T-antigen disaccharide]]
| + | |
| Structural highlights
Function
BEL_BOLED
Publication Abstract from PubMed
A novel lectin has been isolated from the fruiting bodies of the common edible mushroom Boletus edulis (king bolete, penny bun, porcino, or cep) by affinity chromatography on a chitin column. We propose for the lectin the name BEL (Boletus edulis lectin). BEL inhibits selectively the proliferation of several malignant cell lines and binds the neoplastic cell specific T-antigen disaccharide, Galbeta1-3GalNAc. The lectin was structurally characterized: the molecule is a homotetramer and the 142 amino acid sequence of the chains was determined. The protein belongs to the saline-soluble family of mushroom fruiting body specific lectins. BEL was also crystallized and its three-dimensional structure was determined by X-ray diffraction to 1.15 A resolution. The structure is similar to that of Agaricus bisporus lectin. Using the appropriate co-crystals, the interactions of BEL with specific mono and disaccharides were also studied by X-ray diffraction. The six structures of carbohydrate complexes reported here provide details of the interactions of the ligands with the lectin and shed light on the selectivity of the two distinct binding sites present in each protomer.
STRUCTURE OF A LECTIN WITH ANTITUMORAL PROPERTIES IN KING BOLETE (Boletus edulis) MUSHROOMS.,Bovi M, Carrizo ME, Capaldi S, Perduca M, Chiarelli LR, Galliano M, Monaco HL Glycobiology. 2011 Feb 8. PMID:21303815[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bovi M, Carrizo ME, Capaldi S, Perduca M, Chiarelli LR, Galliano M, Monaco HL. STRUCTURE OF A LECTIN WITH ANTITUMORAL PROPERTIES IN KING BOLETE (Boletus edulis) MUSHROOMS. Glycobiology. 2011 Feb 8. PMID:21303815 doi:10.1093/glycob/cwr012
|
