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| | ==The crystal structure of the 5c.c7 TCR== | | ==The crystal structure of the 5c.c7 TCR== |
| - | <StructureSection load='3qjh' size='340' side='right' caption='[[3qjh]], [[Resolution|resolution]] 1.90Å' scene=''> | + | <StructureSection load='3qjh' size='340' side='right'caption='[[3qjh]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3qjh]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QJH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QJH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3qjh]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QJH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QJH FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3qib|3qib]], [[3qiw|3qiw]], [[3qjf|3qjf]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qjh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qjh OCA], [http://pdbe.org/3qjh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3qjh RCSB], [http://www.ebi.ac.uk/pdbsum/3qjh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3qjh ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qjh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qjh OCA], [https://pdbe.org/3qjh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qjh RCSB], [https://www.ebi.ac.uk/pdbsum/3qjh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qjh ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Davis, M M]] | + | [[Category: Mus musculus]] |
| - | [[Category: Ely, L K]] | + | [[Category: Davis MM]] |
| - | [[Category: Garcia, K C]] | + | [[Category: Ely LK]] |
| - | [[Category: Newell, E W]] | + | [[Category: Garcia KC]] |
| - | [[Category: Immune system]] | + | [[Category: Newell EW]] |
| - | [[Category: Immunoglobulin domain]]
| + | |
| - | [[Category: T cell receptor]]
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| Structural highlights
Publication Abstract from PubMed
T cells specific for the cytochrome c Ag are widely used to investigate many aspects of TCR specificity and interactions with peptide-MHC, but structural information has long been elusive. In this study, we present structures for the well-studied 2B4 TCR, as well as a naturally occurring variant of the 5c.c7 TCR, 226, which is cross-reactive with more than half of possible substitutions at all three TCR-sensitive residues on the peptide Ag. These structures alone and in complex with peptide-MHC ligands allow us to reassess many prior mutagenesis results. In addition, the structure of 226 bound to one peptide variant, p5E, shows major changes in the CDR3 contacts compared with wild-type, yet the TCR V-region contacts with MHC are conserved. These and other data illustrate the ability of TCRs to accommodate large variations in CDR3 structure and peptide contacts within the constraints of highly conserved TCR-MHC interactions.
Structural Basis of Specificity and Cross-Reactivity in T Cell Receptors Specific for Cytochrome c-I-E.,Newell EW, Ely LK, Kruse AC, Reay PA, Rodriguez SN, Lin AE, Kuhns MS, Garcia KC, Davis MM J Immunol. 2011 Apr 13. PMID:21490152[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Newell EW, Ely LK, Kruse AC, Reay PA, Rodriguez SN, Lin AE, Kuhns MS, Garcia KC, Davis MM. Structural Basis of Specificity and Cross-Reactivity in T Cell Receptors Specific for Cytochrome c-I-E. J Immunol. 2011 Apr 13. PMID:21490152 doi:10.4049/jimmunol.1100197
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