3imb

<StructureSection load='3imb' size='340' side='right' caption='[[3imb]], [[Resolution|resolution]] 1.89&Aring;' scene=''>

<table><tr><td colspan='2'>[[3imb]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_centrosporus"_ford_1916 "bacillus centrosporus" ford 1916]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IMB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IMB FirstGlance]. <br>

</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2odi|2odi]], [[2odh|2odh]], [[2oaa|2oaa]], [[2oa9|2oa9]], [[2aor|2aor]], [[2azo|2azo]]</td></tr>

<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bcnIR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=54910 "Bacillus centrosporus" Ford 1916])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3imb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3imb OCA], [http://pdbe.org/3imb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3imb RCSB], [http://www.ebi.ac.uk/pdbsum/3imb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3imb ProSAT]</span></td></tr>

<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/im/3imb_consurf.spt"</scriptWhenChecked>

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== Publication Abstract from PubMed ==

Restriction endonuclease BcnI cleaves duplex DNA containing the sequence CC/SGG (S stands for C or G, / designates a cleavage position) to generate staggered products with single nucleotide 5'-overhangs. Here, we show that BcnI functions as a monomer that interacts with its target DNA in 1:1 molar ratio and report crystal structures of BcnI in the absence and in the presence of DNA. In the complex with DNA, BcnI makes specific contacts with all five bases of the target sequence and not just with a half-site, as the protomer of a typical dimeric restriction endonuclease. Our data are inconsistent with BcnI dimerization and suggest that the enzyme introduces double-strand breaks by sequentially nicking individual DNA strands, although this remains to be confirmed by kinetic experiments. BcnI is remotely similar to the DNA repair protein MutH and shares approximately 20% sequence identity with the restriction endonuclease MvaI, which is specific for the related sequence CC/WGG (W stands for A or T). As expected, BcnI is structurally similar to MvaI and recognizes conserved bases in the target sequence similarly but not identically. BcnI has a unique machinery for the recognition of the central base-pair.

Monomeric restriction endonuclease BcnI in the apo form and in an asymmetric complex with target DNA.,Sokolowska M, Kaus-Drobek M, Czapinska H, Tamulaitis G, Szczepanowski RH, Urbanke C, Siksnys V, Bochtler M J Mol Biol. 2007 Jun 8;369(3):722-34. Epub 2007 Mar 15. PMID:17445830<ref>PMID:17445830</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 3imb" style="background-color:#fffaf0;"></div>

== References ==

<references/>

[[Category: Bacillus centrosporus ford 1916]]

[[Category: Bochtler, M]]

[[Category: Czapinska, H]]

[[Category: Kaus-Drobek, M]]

[[Category: Siksnys, V]]

[[Category: Sokolowska, M]]

[[Category: Szczepanowski, R H]]

[[Category: Tamulaitis, G]]

[[Category: Restriction enzyme]]