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1lc6

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[[Image:1lc6.gif|left|200px]]
 
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{{Structure
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==Solution Structure of the U6 Intramolecular Stem-loop RNA==
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|PDB= 1lc6 |SIZE=350|CAPTION= <scene name='initialview01'>1lc6</scene>
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<StructureSection load='1lc6' size='340' side='right'caption='[[1lc6]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
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<table><tr><td colspan='2'>[[1lc6]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LC6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LC6 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lc6 OCA], [https://pdbe.org/1lc6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lc6 RCSB], [https://www.ebi.ac.uk/pdbsum/1lc6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lc6 ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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<div style="background-color:#fffaf0;">
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lc6 OCA], [http://www.ebi.ac.uk/pdbsum/1lc6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lc6 RCSB]</span>
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== Publication Abstract from PubMed ==
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}}
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'''Solution Structure of the U6 Intramolecular Stem-loop RNA'''
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==Overview==
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U6 RNA is a key component of the catalytic core of the spliceosome. A metal ion essential for the first catalytic step of pre-mRNA splicing binds to the U80 Sp phosphate oxygen within the yeast U6 intramolecular stem-loop (ISL). Here we present the first structural data for U6 RNA, revealing the three-dimensional structure of the highly conserved U6 ISL. The ISL binds metal ion at the U80 site with the same stereo specificity as the intact spliceosome. The metal-binding site is adjacent to a readily protonated C.A wobble pair. Protonation of the C.A pair and metal binding are mutually antagonistic. These results support a ribozyme model for U6 RNA function and suggest a possible mechanism for the regulation of RNA splicing.
U6 RNA is a key component of the catalytic core of the spliceosome. A metal ion essential for the first catalytic step of pre-mRNA splicing binds to the U80 Sp phosphate oxygen within the yeast U6 intramolecular stem-loop (ISL). Here we present the first structural data for U6 RNA, revealing the three-dimensional structure of the highly conserved U6 ISL. The ISL binds metal ion at the U80 site with the same stereo specificity as the intact spliceosome. The metal-binding site is adjacent to a readily protonated C.A wobble pair. Protonation of the C.A pair and metal binding are mutually antagonistic. These results support a ribozyme model for U6 RNA function and suggest a possible mechanism for the regulation of RNA splicing.
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==About this Structure==
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Metal binding and base ionization in the U6 RNA intramolecular stem-loop structure.,Huppler A, Nikstad LJ, Allmann AM, Brow DA, Butcher SE Nat Struct Biol. 2002 Jun;9(6):431-5. PMID:11992125<ref>PMID:11992125</ref>
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1LC6 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LC6 OCA].
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==Reference==
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Metal binding and base ionization in the U6 RNA intramolecular stem-loop structure., Huppler A, Nikstad LJ, Allmann AM, Brow DA, Butcher SE, Nat Struct Biol. 2002 Jun;9(6):431-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11992125 11992125]
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[[Category: Protein complex]]
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[[Category: Allmann, A M.]]
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[[Category: Brow, D A.]]
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[[Category: Butcher, S E.]]
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[[Category: Huppler, A.]]
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[[Category: Nikstad, L J.]]
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[[Category: pentaloop]]
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[[Category: rna]]
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[[Category: stem-loop]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:00:56 2008''
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1lc6" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Allmann AM]]
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[[Category: Brow DA]]
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[[Category: Butcher SE]]
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[[Category: Huppler A]]
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[[Category: Nikstad LJ]]

Current revision

Solution Structure of the U6 Intramolecular Stem-loop RNA

PDB ID 1lc6

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