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| - | [[Image:1lci.jpg|left|200px]] | |
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| - | {{Structure
| + | ==FIREFLY LUCIFERASE== |
| - | |PDB= 1lci |SIZE=350|CAPTION= <scene name='initialview01'>1lci</scene>, resolution 2.0Å
| + | <StructureSection load='1lci' size='340' side='right'caption='[[1lci]], [[Resolution|resolution]] 2.00Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND=
| + | <table><tr><td colspan='2'>[[1lci]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Photinus_pyralis Photinus pyralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LCI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LCI FirstGlance]. <br> |
| - | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Photinus-luciferin_4-monooxygenase_(ATP-hydrolyzing) Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.7 1.13.12.7] </span>
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | |GENE=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lci FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lci OCA], [https://pdbe.org/1lci PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lci RCSB], [https://www.ebi.ac.uk/pdbsum/1lci PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lci ProSAT]</span></td></tr> |
| - | |DOMAIN=
| + | </table> |
| - | |RELATEDENTRY= | + | == Function == |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lci FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lci OCA], [http://www.ebi.ac.uk/pdbsum/1lci PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lci RCSB]</span> | + | [https://www.uniprot.org/uniprot/LUCI_PHOPY LUCI_PHOPY] Produces green light with a wavelength of 562 nm. |
| - | }}
| + | == Evolutionary Conservation == |
| | + | [[Image:Consurf_key_small.gif|200px|right]] |
| | + | Check<jmol> |
| | + | <jmolCheckbox> |
| | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lc/1lci_consurf.spt"</scriptWhenChecked> |
| | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | + | <text>to colour the structure by Evolutionary Conservation</text> |
| | + | </jmolCheckbox> |
| | + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lci ConSurf]. |
| | + | <div style="clear:both"></div> |
| | | | |
| - | '''FIREFLY LUCIFERASE'''
| + | ==See Also== |
| - | | + | *[[Luciferase 3D structures|Luciferase 3D structures]] |
| - | | + | __TOC__ |
| - | ==Overview== | + | </StructureSection> |
| - | BACKGROUND. Firefly luciferase is a 62 kDa protein that catalyzes the production of light. In the presence of MgATP and molecular oxygen, the enzyme oxidizes its substrate, firefly luciferin, emitting yellow-green light. The reaction proceeds through activation of the substrate to form an adenylate intermediate. Firefly luciferase shows extensive sequence homology with a number of enzymes that utilize ATP in adenylation reactions. RESULTS. We have determined the crystal structure of firefly luciferase at 2.0 A resolution. The protein is folded into two compact domains. The large N-terminal domain consists of a beta-barrel and two beta-sheets. The sheets are flanked by alpha-helices to form an alphabetaalphabetaalpha five-layered structure. The C-terminal portion of the molecule forms a distinct domain, which is separated from the N-terminal domain by a wide cleft. CONCLUSIONS. Firefly luciferase is the first member of a superfamily of homologous enzymes, which includes acyl-coenzyme A ligases and peptide synthetases, to have its structure characterized. The residues conserved within the superfamily are located on the surfaces of the two domains on either side of the cleft, but are too far apart to interact simultaneously with the substrates. This suggests that the two domains will close in the course of the reaction. Firefly luciferase has a novel structural framework for catalyzing adenylate-forming reactions.
| + | [[Category: Large Structures]] |
| - | | + | |
| - | ==About this Structure==
| + | |
| - | 1LCI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Photinus_pyralis Photinus pyralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LCI OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes., Conti E, Franks NP, Brick P, Structure. 1996 Mar 15;4(3):287-98. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8805533 8805533]
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| | [[Category: Photinus pyralis]] | | [[Category: Photinus pyralis]] |
| - | [[Category: Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing)]]
| + | [[Category: Brick P]] |
| - | [[Category: Single protein]]
| + | [[Category: Conti E]] |
| - | [[Category: Brick, P.]] | + | [[Category: Franks NP]] |
| - | [[Category: Conti, E.]] | + | |
| - | [[Category: Franks, N P.]] | + | |
| - | [[Category: luminescence]]
| + | |
| - | [[Category: monooxygenase]]
| + | |
| - | [[Category: oxidoreductase]]
| + | |
| - | [[Category: photoprotein]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:01:08 2008''
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