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3wdn

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High-resolution X-ray crystal structure of bovine H-protein using a high-pressure cryocooling method

Structural highlights

3wdn is a 1 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 0.86Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GCSH_BOVIN The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.

Publication Abstract from PubMed

Recently, many technical improvements in macromolecular X-ray crystallography have increased the number of structures deposited in the Protein Data Bank and improved the resolution limit of protein structures. Almost all high-resolution structures have been determined using a synchrotron radiation source in conjunction with cryocooling techniques, which are required in order to minimize radiation damage. However, optimization of cryoprotectant conditions is a time-consuming and difficult step. To overcome this problem, the high-pressure cryocooling method was developed (Kim et al., 2005) and successfully applied to many protein-structure analyses. In this report, using the high-pressure cryocooling method, the X-ray crystal structure of bovine H-protein was determined at 0.86 A resolution. Structural comparisons between high- and ambient-pressure cryocooled crystals at ultra-high resolution illustrate the versatility of this technique. This is the first ultra-high-resolution X-ray structure obtained using the high-pressure cryocooling method.

High-resolution X-ray crystal structure of bovine H-protein using the high-pressure cryocooling method.,Higashiura A, Ohta K, Masaki M, Sato M, Inaka K, Tanaka H, Nakagawa A J Synchrotron Radiat. 2013 Nov;20(Pt 6):989-93. doi: 10.1107/S090904951302373X., Epub 2013 Oct 5. PMID:24121354^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Higashiura A, Ohta K, Masaki M, Sato M, Inaka K, Tanaka H, Nakagawa A. High-resolution X-ray crystal structure of bovine H-protein using the high-pressure cryocooling method. J Synchrotron Radiat. 2013 Nov;20(Pt 6):989-93. doi: 10.1107/S090904951302373X., Epub 2013 Oct 5. PMID:24121354 doi:http://dx.doi.org/10.1107/S090904951302373X

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3wdn"

Categories: Bos taurus | Large Structures | Higashiura A | Nakagawa A

@@ Line 1: / Line 1: @@
 ==High-resolution X-ray crystal structure of bovine H-protein using a high-pressure cryocooling method==
-<StructureSection load='3wdn' size='340' side='right' caption='[[3wdn]], [[Resolution|resolution]] 0.86&Aring;' scene=''>
+<StructureSection load='3wdn' size='340' side='right'caption='[[3wdn]], [[Resolution|resolution]] 0.86&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3wdn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WDN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WDN FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3wdn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WDN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WDN FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.86&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GCSH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wdn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wdn OCA], [http://pdbe.org/3wdn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wdn RCSB], [http://www.ebi.ac.uk/pdbsum/3wdn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wdn ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wdn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wdn OCA], [https://pdbe.org/3wdn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wdn RCSB], [https://www.ebi.ac.uk/pdbsum/3wdn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wdn ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/GCSH_BOVIN GCSH_BOVIN]] The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.
+[https://www.uniprot.org/uniprot/GCSH_BOVIN GCSH_BOVIN] The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Bovin]]
+[[Category: Bos taurus]]
-[[Category: Higashiura, A]]
+[[Category: Large Structures]]
-[[Category: Nakagawa, A]]
+[[Category: Higashiura A]]
-[[Category: Antiparallel beta sheet]]
+[[Category: Nakagawa A]]
-[[Category: Beta sandwich]]
-[[Category: Oxidoreductase]]

3wdn

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Current revision

High-resolution X-ray crystal structure of bovine H-protein using a high-pressure cryocooling method

Structural highlights

Function

Publication Abstract from PubMed

References

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Proteopedia Page Contributors and Editors (what is this?)

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