This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

3vmt

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Current revision

Crystal structure of Staphylococcus aureus membrane-bound transglycosylase in complex with a Lipid II analog

Structural highlights

3vmt is a 2 chain structure with sequence from Staphylococcus aureus subsp. aureus Mu50. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.299Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MGT_STAAM Involved in the biosynthesis of cell wall peptidoglycan. Responsible for the elongation of the glycan strands using lipid-linked disaccharide-pentapeptide as the substrate (By similarity).

Publication Abstract from PubMed

Bacterial transpeptidase and transglycosylase on the surface are essential for cell wall synthesis, and many antibiotics have been developed to target the transpeptidase; however, the problem of antibiotic resistance has arisen and caused a major threat in bacterial infection. The transglycosylase has been considered to be another excellent target, but no antibiotics have been developed to target this enzyme. Here, we determined the crystal structure of the Staphylococcus aureus membrane-bound transglycosylase, monofunctional glycosyltransferase, in complex with a lipid II analog to 2.3 A resolution. Our results showed that the lipid II-contacting residues are not only conserved in WT and drug-resistant bacteria but also significant in enzymatic activity. Mechanistically, we proposed that K140 and R148 in the donor site, instead of the previously proposed E156, are used to stabilize the pyrophosphate-leaving group of lipid II, and E100 in the acceptor site acts as general base for the 4-OH of GlcNAc to facilitate the transglycosylation reaction. This mechanism, further supported by mutagenesis study and the structure of monofunctional glycosyltransferase in complex with moenomycin in the donor site, provides a direction for antibacterial drugs design.

Crystal structure of Staphylococcus aureus transglycosylase in complex with a lipid II analog and elucidation of peptidoglycan synthesis mechanism.,Huang CY, Shih HW, Lin LY, Tien YW, Cheng TJ, Cheng WC, Wong CH, Ma C Proc Natl Acad Sci U S A. 2012 Apr 24;109(17):6496-501. Epub 2012 Apr 9. PMID:22493270^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Huang CY, Shih HW, Lin LY, Tien YW, Cheng TJ, Cheng WC, Wong CH, Ma C. Crystal structure of Staphylococcus aureus transglycosylase in complex with a lipid II analog and elucidation of peptidoglycan synthesis mechanism. Proc Natl Acad Sci U S A. 2012 Apr 24;109(17):6496-501. Epub 2012 Apr 9. PMID:22493270 doi:10.1073/pnas.1203900109

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3vmt"

@@ Line 1: / Line 1: @@
 ==Crystal structure of Staphylococcus aureus membrane-bound transglycosylase in complex with a Lipid II analog==
-<StructureSection load='3vmt' size='340' side='right' caption='[[3vmt]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+<StructureSection load='3vmt' size='340' side='right'caption='[[3vmt]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3vmt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staam Staam]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VMT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VMT FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3vmt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VMT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VMT FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LHI:[(2R,3R,4R,5S,6R)-4-[(2R)-1-[[(2S)-1-[2-[2-[2-[5-[(3AS,4S,6AR)-2-OXIDANYLIDENE-1,3,3A,4,6,6A-HEXAHYDROTHIENO[3,4-D]IMIDAZOL-4-YL]PENTANOYLAMINO]ETHOXY]ETHOXY]ETHYLAMINO]-1-OXIDANYLIDENE-PROPAN-2-YL]AMINO]-1-OXIDANYLIDENE-PROPAN-2-YL]OXY-3-ACETAMIDO-5-[(2S,3R,4R,5R,6R)-3-ACETAMIDO-6-(HYDROXYMETHYL)-4,5-BIS(OXIDANYL)OXAN-2-YL]OXY-6-(HYDROXYMETHYL)OXAN-2-YL]+[OXIDANYL(3,7,11,15,19,23,27,31,35,39,43-UNDECAMETHYLTETRATETRACONTA-2,6,10,14,18,22,26,30,34,38,42-UNDECAENOXY)PHOSPHORYL]+HYDROGEN+PHOSPHATE'>LHI</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.299&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3vmq|3vmq]], [[3vmr|3vmr]], [[3vms|3vms]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LHI:[(2R,3R,4R,5S,6R)-4-[(2R)-1-[[(2S)-1-[2-[2-[2-[5-[(3AS,4S,6AR)-2-OXIDANYLIDENE-1,3,3A,4,6,6A-HEXAHYDROTHIENO[3,4-D]IMIDAZOL-4-YL]PENTANOYLAMINO]ETHOXY]ETHOXY]ETHYLAMINO]-1-OXIDANYLIDENE-PROPAN-2-YL]AMINO]-1-OXIDANYLIDENE-PROPAN-2-YL]OXY-3-ACETAMIDO-5-[(2S,3R,4R,5R,6R)-3-ACETAMIDO-6-(HYDROXYMETHYL)-4,5-BIS(OXIDANYL)OXAN-2-YL]OXY-6-(HYDROXYMETHYL)OXAN-2-YL]+[OXIDANYL(3,7,11,15,19,23,27,31,35,39,43-UNDECAMETHYLTETRATETRACONTA-2,6,10,14,18,22,26,30,34,38,42-UNDECAENOXY)PHOSPHORYL]+HYDROGEN+PHOSPHATE'>LHI</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MGT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 STAAM])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vmt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vmt OCA], [https://pdbe.org/3vmt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vmt RCSB], [https://www.ebi.ac.uk/pdbsum/3vmt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vmt ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vmt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vmt OCA], [http://pdbe.org/3vmt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vmt RCSB], [http://www.ebi.ac.uk/pdbsum/3vmt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vmt ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/MGT_STAAM MGT_STAAM]] Involved in the biosynthesis of cell wall peptidoglycan. Responsible for the elongation of the glycan strands using lipid-linked disaccharide-pentapeptide as the substrate (By similarity).
+[https://www.uniprot.org/uniprot/MGT_STAAM MGT_STAAM] Involved in the biosynthesis of cell wall peptidoglycan. Responsible for the elongation of the glycan strands using lipid-linked disaccharide-pentapeptide as the substrate (By similarity).
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 21: @@
 ==See Also==
-*[[Glycosyltransferase|Glycosyltransferase]]
+*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Staam]]
+[[Category: Large Structures]]
-[[Category: Cheng, T J.R]]
+[[Category: Staphylococcus aureus subsp. aureus Mu50]]
-[[Category: Cheng, W C]]
+[[Category: Cheng TJR]]
-[[Category: Huang, C Y]]
+[[Category: Cheng WC]]
-[[Category: Lin, L Y]]
+[[Category: Huang CY]]
-[[Category: Ma, C]]
+[[Category: Lin LY]]
-[[Category: Shih, H W]]
+[[Category: Ma C]]
-[[Category: Tien, Y W]]
+[[Category: Shih HW]]
-[[Category: Wong, C H]]
+[[Category: Tien YW]]
-[[Category: Bacterial cell wall synthesis]]
+[[Category: Wong CH]]
-[[Category: Glycosyltransferase]]
-[[Category: Membrane]]
-[[Category: Transferase]]
-[[Category: Transmembrane]]

3vmt

From Proteopedia

Current revision

Crystal structure of Staphylococcus aureus membrane-bound transglycosylase in complex with a Lipid II analog

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox