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| - | ==STRUCTURE OF THE TBL1 TETRAMERISATION DOMAIN== | + | ==Structure of the TBL1 tetramerisation domain== |
| - | <StructureSection load='2xtc' size='340' side='right' caption='[[2xtc]], [[Resolution|resolution]] 2.22Å' scene=''> | + | <StructureSection load='2xtc' size='340' side='right'caption='[[2xtc]], [[Resolution|resolution]] 2.22Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2xtc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XTC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XTC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2xtc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XTC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XTC FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2xte|2xte]], [[2xtd|2xtd]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.22Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xtc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xtc OCA], [http://pdbe.org/2xtc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xtc RCSB], [http://www.ebi.ac.uk/pdbsum/2xtc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xtc ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xtc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xtc OCA], [https://pdbe.org/2xtc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xtc RCSB], [https://www.ebi.ac.uk/pdbsum/2xtc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xtc ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/TBL1X_HUMAN TBL1X_HUMAN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Fairall, L]] | + | [[Category: Large Structures]] |
| - | [[Category: Greenwood, J A]] | + | [[Category: Fairall L]] |
| - | [[Category: Oberoi, J]] | + | [[Category: Greenwood JA]] |
| - | [[Category: Schwabe, J W.R]] | + | [[Category: Oberoi J]] |
| - | [[Category: Watson, P J]] | + | [[Category: Schwabe JWR]] |
| - | [[Category: Transcription]] | + | [[Category: Watson PJ]] |
| Structural highlights
Function
TBL1X_HUMAN
Publication Abstract from PubMed
Eukaryotic transcriptional repressors function by recruiting large coregulatory complexes that target histone deacetylase enzymes to gene promoters and enhancers. Transcriptional repression complexes, assembled by the corepressor NCoR and its homolog SMRT, are crucial in many processes, including development and metabolic physiology. The core repression complex involves the recruitment of three proteins, HDAC3, GPS2 and TBL1, to a highly conserved repression domain within SMRT and NCoR. We have used structural and functional approaches to gain insight into the architecture and biological role of this complex. We report the crystal structure of the tetrameric oligomerization domain of TBL1, which interacts with both SMRT and GPS2, and the NMR structure of the interface complex between GPS2 and SMRT. These structures, together with computational docking, mutagenesis and functional assays, reveal the assembly mechanism and stoichiometry of the corepressor complex.
Structural basis for the assembly of the SMRT/NCoR core transcriptional repression machinery.,Oberoi J, Fairall L, Watson PJ, Yang JC, Czimmerer Z, Kampmann T, Goult BT, Greenwood JA, Gooch JT, Kallenberger BC, Nagy L, Neuhaus D, Schwabe JW Nat Struct Mol Biol. 2011 Jan 16. PMID:21240272[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Oberoi J, Fairall L, Watson PJ, Yang JC, Czimmerer Z, Kampmann T, Goult BT, Greenwood JA, Gooch JT, Kallenberger BC, Nagy L, Neuhaus D, Schwabe JW. Structural basis for the assembly of the SMRT/NCoR core transcriptional repression machinery. Nat Struct Mol Biol. 2011 Jan 16. PMID:21240272 doi:10.1038/nsmb.1983
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