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| | ==The crystal structure of hemoglobin from woolly mammoth in the carbonmonoxy forms== | | ==The crystal structure of hemoglobin from woolly mammoth in the carbonmonoxy forms== |
| - | <StructureSection load='3vrf' size='340' side='right' caption='[[3vrf]], [[Resolution|resolution]] 1.55Å' scene=''> | + | <StructureSection load='3vrf' size='340' side='right'caption='[[3vrf]], [[Resolution|resolution]] 1.55Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3vrf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mampr Mampr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VRF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VRF FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3vrf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mammuthus_primigenius Mammuthus primigenius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VRF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VRF FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3vfe|3vfe]], [[3vfg|3vfg]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HBA-T2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=37349 MAMPR]), HBB/D ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=37349 MAMPR])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vrf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vrf OCA], [https://pdbe.org/3vrf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vrf RCSB], [https://www.ebi.ac.uk/pdbsum/3vrf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vrf ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vrf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vrf OCA], [http://pdbe.org/3vrf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vrf RCSB], [http://www.ebi.ac.uk/pdbsum/3vrf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vrf ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/D3U1H8_MAMPR D3U1H8_MAMPR] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Mampr]] | + | [[Category: Large Structures]] |
| - | [[Category: Campbell, K L]] | + | [[Category: Mammuthus primigenius]] |
| - | [[Category: Ho, C]] | + | [[Category: Campbell KL]] |
| - | [[Category: Noguchi, H]] | + | [[Category: Ho C]] |
| - | [[Category: Park, S Y]] | + | [[Category: Noguchi H]] |
| - | [[Category: Tame, J R.H]] | + | [[Category: Park S-Y]] |
| - | [[Category: Oxygen storage/transport]]
| + | [[Category: Tame JRH]] |
| - | [[Category: Oxygen transport]]
| + | |
| - | [[Category: Woolly mammoth hemoglobin]]
| + | |
| Structural highlights
Function
D3U1H8_MAMPR
Publication Abstract from PubMed
The haemoglobin (Hb) of the extinct woolly mammoth has been recreated using recombinant genes expressed in Escherichia coli. The globin gene sequences were previously determined using DNA recovered from frozen cadavers. Although highly similar to the Hb of existing elephants, the woolly mammoth protein shows rather different responses to chloride ions and temperature. In particular, the heat of oxygenation is found to be much lower in mammoth Hb, which appears to be an adaptation to the harsh high-latitude climates of the Pleistocene Ice Ages and has been linked to heightened sensitivity of the mammoth protein to protons, chloride ions and organic phosphates relative to that of Asian elephants. To elucidate the structural basis for the altered homotropic and heterotropic effects, the crystal structures of mammoth Hb have been determined in the deoxy, carbonmonoxy and aquo-met forms. These models, which are the first structures of Hb from an extinct species, show many features reminiscent of human Hb, but underline how the delicate control of oxygen affinity relies on much more than simple overall quaternary-structure changes.
Structures of haemoglobin from woolly mammoth in liganded and unliganded states.,Noguchi H, Campbell KL, Ho C, Unzai S, Park SY, Tame JR Acta Crystallogr D Biol Crystallogr. 2012 Nov;68(Pt 11):1441-9. doi:, 10.1107/S0907444912029459. Epub 2012 Oct 18. PMID:23090393[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Noguchi H, Campbell KL, Ho C, Unzai S, Park SY, Tame JR. Structures of haemoglobin from woolly mammoth in liganded and unliganded states. Acta Crystallogr D Biol Crystallogr. 2012 Nov;68(Pt 11):1441-9. doi:, 10.1107/S0907444912029459. Epub 2012 Oct 18. PMID:23090393 doi:http://dx.doi.org/10.1107/S0907444912029459
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