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| | ==Crystal Structure of the Atg17-Atg31-Atg29 Complex== | | ==Crystal Structure of the Atg17-Atg31-Atg29 Complex== |
| - | <StructureSection load='4hpq' size='340' side='right' caption='[[4hpq]], [[Resolution|resolution]] 3.06Å' scene=''> | + | <StructureSection load='4hpq' size='340' side='right'caption='[[4hpq]], [[Resolution|resolution]] 3.06Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4hpq]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_thermotolerans Kluyveromyces thermotolerans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HPQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HPQ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4hpq]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Lachancea_thermotolerans_CBS_6340 Lachancea thermotolerans CBS 6340]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HPQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HPQ FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.06Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Atg31, KLTH0C07942g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559295 Kluyveromyces thermotolerans]), Atg29, KLTH0D11660g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559295 Kluyveromyces thermotolerans]), Atg17, KLTH0D15642g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559295 Kluyveromyces thermotolerans])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hpq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hpq OCA], [https://pdbe.org/4hpq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hpq RCSB], [https://www.ebi.ac.uk/pdbsum/4hpq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hpq ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hpq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hpq OCA], [http://pdbe.org/4hpq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hpq RCSB], [http://www.ebi.ac.uk/pdbsum/4hpq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hpq ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/C5DFJ6_LACTC C5DFJ6_LACTC] |
| - | Macroautophagy is a bulk clearance mechanism in which the double-membraned phagophore grows and engulfs cytosolic material. In yeast, the phagophore nucleates from a cluster of 20-30 nm diameter Atg9-containing vesicles located at a multiprotein assembly known as the preautophagosomal structure (PAS). The crystal structure of a 2:2:2 complex of the earliest acting PAS proteins, Atg17, Atg29, and Atg31, was solved at 3.05 A resolution. Atg17 is crescent shaped with a 10 nm radius of curvature. Dimerization of the Atg17-Atg31-Atg29 complex is critical for both PAS formation and autophagy, and each dimer contains two separate and complete crescents. Upon induction of autophagy, Atg17-Atg31-Atg29 assembles with Atg1 and Atg13, which in turn initiates the formation of the phagophore. The C-terminal EAT domain of Atg1 was shown to sense membrane curvature, dimerize, and tether lipid vesicles. These data suggest a structural mechanism for the organization of Atg9 vesicles into the early phagophore.
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| - | Architecture of the atg17 complex as a scaffold for autophagosome biogenesis.,Ragusa MJ, Stanley RE, Hurley JH Cell. 2012 Dec 21;151(7):1501-12. doi: 10.1016/j.cell.2012.11.028. Epub 2012 Dec , 6. PMID:23219485<ref>PMID:23219485</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 4hpq" style="background-color:#fffaf0;"></div>
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| | ==See Also== | | ==See Also== |
| - | *[[Autophagy-related protein|Autophagy-related protein]] | + | *[[Autophagy-related protein 3D structures|Autophagy-related protein 3D structures]] |
| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Kluyveromyces thermotolerans]] | + | [[Category: Lachancea thermotolerans CBS 6340]] |
| - | [[Category: Hurley, J H]] | + | [[Category: Large Structures]] |
| - | [[Category: Ragusa, M J]] | + | [[Category: Hurley JH]] |
| - | [[Category: Stanley, R E]] | + | [[Category: Ragusa MJ]] |
| - | [[Category: Autophagy]] | + | [[Category: Stanley RE]] |
| - | [[Category: Protein transport]]
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