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4gd9

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==Circular Permuted Streptavidin N49/G48==
==Circular Permuted Streptavidin N49/G48==
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<StructureSection load='4gd9' size='340' side='right' caption='[[4gd9]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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<StructureSection load='4gd9' size='340' side='right'caption='[[4gd9]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4gd9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GD9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GD9 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4gd9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GD9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GD9 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1swf|1swf]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gd9 OCA], [http://pdbe.org/4gd9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gd9 RCSB], [http://www.ebi.ac.uk/pdbsum/4gd9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gd9 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gd9 OCA], [https://pdbe.org/4gd9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gd9 RCSB], [https://www.ebi.ac.uk/pdbsum/4gd9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gd9 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
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[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Circular permutation of streptavidin was carried out in order to investigate the role of a main-chain amide in stabilizing the high-affinity complex of the protein and biotin. Mutant proteins CP49/48 and CP50/49 were constructed to place new N-termini at residues 49 and 50 in a flexible loop involved in stabilizing the biotin complex. Crystal structures of the two mutants show that half of each loop closes over the binding site, as observed in wild-type streptavidin, while the other half adopts the open conformation found in the unliganded state. The structures are consistent with kinetic and thermodynamic data and indicate that the loop plays a role in enthalpic stabilization of the bound state via the Asn49 amide-biotin hydrogen bond. In wild-type streptavidin, the entropic penalties of immobilizing a flexible portion of the protein to enhance binding are kept to a manageable level by using a contiguous loop of medium length (six residues) which is already constrained by its anchorage to strands of the beta-barrel protein. A molecular-dynamics simulation for CP50/49 shows that cleavage of the binding loop results in increased structural fluctuations for Ser45 and that these fluctuations destabilize the streptavidin-biotin complex.
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Structural consequences of cutting a binding loop: two circularly permuted variants of streptavidin.,Le Trong I, Chu V, Xing Y, Lybrand TP, Stayton PS, Stenkamp RE Acta Crystallogr D Biol Crystallogr. 2013 Jun;69(Pt 6):968-77. doi:, 10.1107/S0907444913003855. Epub 2013 May 11. PMID:23695241<ref>PMID:23695241</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4gd9" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[Avidin|Avidin]]
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*[[Avidin 3D structures|Avidin 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: As 4 1583]]
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[[Category: Large Structures]]
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[[Category: Chu, V]]
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[[Category: Streptomyces avidinii]]
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[[Category: Lybrand, T P]]
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[[Category: Chu V]]
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[[Category: Stayton, P S]]
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[[Category: Le Trong I]]
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[[Category: Stenkamp, R E]]
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[[Category: Lybrand TP]]
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[[Category: Trong, I Le]]
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[[Category: Stayton PS]]
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[[Category: Xing, Y]]
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[[Category: Stenkamp RE]]
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[[Category: Biotin]]
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[[Category: Xing Y]]
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[[Category: Biotin-binding protein]]
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[[Category: Circular permutation]]
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Current revision

Circular Permuted Streptavidin N49/G48

PDB ID 4gd9

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