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| | ==Structural and mutational analysis reveals that CTNNBL1 binds NLSs in a manner distinct from that of its closest armadillo-relative, karyopherin alpha== | | ==Structural and mutational analysis reveals that CTNNBL1 binds NLSs in a manner distinct from that of its closest armadillo-relative, karyopherin alpha== |
| - | <StructureSection load='4cb8' size='340' side='right' caption='[[4cb8]], [[Resolution|resolution]] 2.90Å' scene=''> | + | <StructureSection load='4cb8' size='340' side='right'caption='[[4cb8]], [[Resolution|resolution]] 2.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4cb8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CB8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CB8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4cb8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CB8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CB8 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4cb9|4cb9]], [[4cba|4cba]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cb8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cb8 OCA], [http://pdbe.org/4cb8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cb8 RCSB], [http://www.ebi.ac.uk/pdbsum/4cb8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cb8 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cb8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cb8 OCA], [https://pdbe.org/4cb8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cb8 RCSB], [https://www.ebi.ac.uk/pdbsum/4cb8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cb8 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CTBL1_HUMAN CTBL1_HUMAN]] Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. Participates in AID/AICDA-mediated Ig class switching recombination (CSR). May induce apoptosis.<ref>PMID:12659813</ref> <ref>PMID:18722174</ref> | + | [https://www.uniprot.org/uniprot/CTBL1_HUMAN CTBL1_HUMAN] Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. Participates in AID/AICDA-mediated Ig class switching recombination (CSR). May induce apoptosis.<ref>PMID:12659813</ref> <ref>PMID:18722174</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Ganesh, K]] | + | [[Category: Large Structures]] |
| - | [[Category: Johnson, C M]] | + | [[Category: Ganesh K]] |
| - | [[Category: Neuberger, M S]] | + | [[Category: Johnson CM]] |
| - | [[Category: Rada, C]] | + | [[Category: Neuberger MS]] |
| - | [[Category: Simpson, P]] | + | [[Category: Rada C]] |
| - | [[Category: Telerman, S B]] | + | [[Category: Simpson P]] |
| - | [[Category: Williams, R L]] | + | [[Category: Telerman SB]] |
| - | [[Category: VanMaldegem, F]] | + | [[Category: Williams RL]] |
| - | [[Category: Aid]]
| + | [[Category: VanMaldegem F]] |
| - | [[Category: Apoptosis]]
| + | |
| - | [[Category: Deaminase]]
| + | |
| - | [[Category: Import]]
| + | |
| Structural highlights
Function
CTBL1_HUMAN Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. Participates in AID/AICDA-mediated Ig class switching recombination (CSR). May induce apoptosis.[1] [2]
Publication Abstract from PubMed
CTNNBL1 is a spliceosome-associated protein that binds nuclear localization signals (NLSs) in splice factors CDC5L and Prp31 as well as the antibody diversifying enzyme AID. Here, crystal structures of human CTNNBL1 reveal a distinct structure from its closest homologue karyopherin-alphas. CTNNBL1 comprises a HEAT-like domain (including a nuclear export signal), a central armadillo domain, and a coiled-coil C-terminal domain. Structure-guided mutations of the region homologous to the karyopherin-alpha NLS-binding site fail to disrupt CTNNBL1-NLS interactions. Our results identify CTNNBL1 as a unique selective NLS-binding protein with striking differences from karyopherin-alphas.
Structural and mutational analysis reveals that CTNNBL1 binds NLSs in a manner distinct from that of its closest armadillo-relative, karyopherin alpha,Ganesh K, van Maldegem F, Telerman SB, Simpson P, Johnson CM, Williams RL, Neuberger MS, Rada C FEBS Lett. 2013 Nov 20. pii: S0014-5793(13)00847-8. doi:, 10.1016/j.febslet.2013.11.013. PMID:24269683[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Jabbour L, Welter JF, Kollar J, Hering TM. Sequence, gene structure, and expression pattern of CTNNBL1, a minor-class intron-containing gene--evidence for a role in apoptosis. Genomics. 2003 Mar;81(3):292-303. PMID:12659813
- ↑ Conticello SG, Ganesh K, Xue K, Lu M, Rada C, Neuberger MS. Interaction between antibody-diversification enzyme AID and spliceosome-associated factor CTNNBL1. Mol Cell. 2008 Aug 22;31(4):474-84. doi: 10.1016/j.molcel.2008.07.009. PMID:18722174 doi:10.1016/j.molcel.2008.07.009
- ↑ Ganesh K, van Maldegem F, Telerman SB, Simpson P, Johnson CM, Williams RL, Neuberger MS, Rada C. Structural and mutational analysis reveals that CTNNBL1 binds NLSs in a manner distinct from that of its closest armadillo-relative, karyopherin alpha FEBS Lett. 2013 Nov 20. pii: S0014-5793(13)00847-8. doi:, 10.1016/j.febslet.2013.11.013. PMID:24269683 doi:http://dx.doi.org/10.1016/j.febslet.2013.11.013
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