422d

<StructureSection load='422d' size='340' side='right' caption='[[422d]], [[Resolution|resolution]] 2.60&Aring;' scene=''>

<table><tr><td colspan='2'>[[422d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=422D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=422D FirstGlance]. <br>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=422d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=422d OCA], [http://pdbe.org/422d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=422d RCSB], [http://www.ebi.ac.uk/pdbsum/422d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=422d ProSAT]</span></td></tr>

<div style="background-color:#fffaf0;">

== Publication Abstract from PubMed ==

The crystal structure of an RNA dodecamer, r(GAUCACUUCGGU), was solved at 2.6 A resolution by the molecular-replacement method and refined to an R(work) of 18.8% (R(free) = 22.8%) using 2494 reflections. The dodecamer crystallized in the monoclinic space group C2, with unit-cell parameters a = 71.34, b = 39.98, c = 32.47 A, beta = 104.7 degrees and two independent strands in the asymmetric unit. The dodecamer adopts an octamer duplex structure with four 5'-overhang residues (G1A2U3C4), which form Watson-Crick base pairs with another four 5'-overhang residues of a symmetry-related duplex. The octamer duplex (ACUUCGGU) contains at its center four mismatched base pairs flanked by two Watson-Crick base pairs. The mismatched bases form two G.U wobble base pairs at the ends and two U.C base pairs at the center, with one base-base hydrogen bond N4(C).O4(U) and a water bridge connecting the N(3) of the cytosine and uridine. The present study reinforces the concept of the stability of the conformation of UUCG in RNA double-helical structures.

Structure of the dodecamer r(GAUCACUUCGGU) with four 5'-overhang nucleotides.,Eswaramoorthy S, Rao ST, Pan B, Sundaralingam M Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):8-12. Epub 2003 Dec, 18. PMID:14684886<ref>PMID:14684886</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

</div>

<div class="pdbe-citations 422d" style="background-color:#fffaf0;"></div>

== References ==

<references/>

[[Category: Eswaramoorthy, S]]

[[Category: Rao, S T]]

[[Category: Sundaralingam, M]]

[[Category: Double helix]]

[[Category: Rna]]