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3q7g

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==Crystal Structure of E2 domain of Human Amyloid Precursor-Like Protein 1 in complex with SOS (sucrose octasulfate)==
==Crystal Structure of E2 domain of Human Amyloid Precursor-Like Protein 1 in complex with SOS (sucrose octasulfate)==
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<StructureSection load='3q7g' size='340' side='right' caption='[[3q7g]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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<StructureSection load='3q7g' size='340' side='right'caption='[[3q7g]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3q7g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q7G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3Q7G FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3q7g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q7G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q7G FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SCR:SUCROSE+OCTASULFATE'>SCR</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3q7l|3q7l]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GU4:2,3,4,6-TETRA-O-SULFONATO-ALPHA-D-GLUCOPYRANOSE'>GU4</scene>, <scene name='pdbligand=PRD_900013:sucrose+octasulfate'>PRD_900013</scene>, <scene name='pdbligand=YYJ:1,3,4,6-tetra-O-sulfo-beta-D-fructofuranose'>YYJ</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">APLP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q7g OCA], [https://pdbe.org/3q7g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q7g RCSB], [https://www.ebi.ac.uk/pdbsum/3q7g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q7g ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3q7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q7g OCA], [http://pdbe.org/3q7g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3q7g RCSB], [http://www.ebi.ac.uk/pdbsum/3q7g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3q7g ProSAT]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/APLP1_HUMAN APLP1_HUMAN]] May play a role in postsynaptic function. The C-terminal gamma-secretase processed fragment, ALID1, activates transcription activation through APBB1 (Fe65) binding (By similarity). Couples to JIP signal transduction through C-terminal binding. May interact with cellular G-protein signaling pathways. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I. The gamma-CTF peptide, C30, is a potent enhancer of neuronal apoptosis.
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[https://www.uniprot.org/uniprot/APLP1_HUMAN APLP1_HUMAN] May play a role in postsynaptic function. The C-terminal gamma-secretase processed fragment, ALID1, activates transcription activation through APBB1 (Fe65) binding (By similarity). Couples to JIP signal transduction through C-terminal binding. May interact with cellular G-protein signaling pathways. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I. The gamma-CTF peptide, C30, is a potent enhancer of neuronal apoptosis.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Missense mutations in the APP gene can cause familial Alzheimer's disease. It is thought that APP and APP-like proteins (APLPs) may play a role in adhesion and signal transduction because their ectodomains interact with components of the extracellular matrix. Heparin binding induces dimerization of APP and APLPs. To help explain how these proteins interact with heparin, we have determined the crystal structure of the E2 domain of APLP1 in complex with sucrose octasulfate (SOS). A total of three SOS molecules are bound to the E2 dimer. Two SOSs are bound inside a narrow inter-subdomain groove, and the third SOS is bound near the protein's 2-fold axis. Mutational analyses show that most residues interacting with SOS also contribute to heparin binding, although in varying degrees: a deep pocket, defined by His-376, Lys-422 and Arg-429, and an interfacial site between Lys-314 and its symmetry mate, are most important in the binding of the negatively charged polysaccharide. Comparison with a lower resolution APP structure shows that all key heparin binding residues are conserved and identically positioned, suggesting that APLP1 and APP may bind heparin similarly. In transfected HEK-293 cells, mutating residues responsible for heparin binding causes little change in the proteolysis of APP by the secretases. However, mutating a pair of conserved basic residues (equivalent to Arg-414 and Arg-415 of APLP1) immediately adjacent to the heparin binding site affects both the maturation and processing of APP.
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The crystal structure of the E2 domain of amyloid precursor protein-like protein 1 in complex with sucrose octasulfate.,Xue Y, Lee S, Wang Y, Ha Y J Biol Chem. 2011 Jun 29. PMID:21715329<ref>PMID:21715329</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3q7g" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Human]]
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[[Category: Homo sapiens]]
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[[Category: Ha, Y]]
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[[Category: Large Structures]]
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[[Category: Xue, Y]]
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[[Category: Ha Y]]
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[[Category: Alzheimer's disease]]
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[[Category: Xue Y]]
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[[Category: Amyloid beta precursor protein]]
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[[Category: E2 domain]]
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[[Category: Heparin]]
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[[Category: Signaling]]
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[[Category: Signaling protein]]
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Current revision

Crystal Structure of E2 domain of Human Amyloid Precursor-Like Protein 1 in complex with SOS (sucrose octasulfate)

PDB ID 3q7g

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