1lmv

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[[Image:1lmv.jpg|left|200px]]
 
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{{Structure
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==Solution structure of the unmodified U2 snRNA-intron branch site helix from S. cerevisiae==
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|PDB= 1lmv |SIZE=350|CAPTION= <scene name='initialview01'>1lmv</scene>
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<StructureSection load='1lmv' size='340' side='right'caption='[[1lmv]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
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<table><tr><td colspan='2'>[[1lmv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LMV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LMV FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lmv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lmv OCA], [https://pdbe.org/1lmv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lmv RCSB], [https://www.ebi.ac.uk/pdbsum/1lmv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lmv ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=[[1lpw|1LPW]]
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<div style="background-color:#fffaf0;">
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lmv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lmv OCA], [http://www.ebi.ac.uk/pdbsum/1lmv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lmv RCSB]</span>
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== Publication Abstract from PubMed ==
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}}
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'''Solution structure of the unmodified U2 snRNA-intron branch site helix from S. cerevisiae'''
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==Overview==
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Pairing of a consensus sequence of the precursor (pre)-mRNA intron with a short region of the U2 small nuclear (sn)RNA during assembly of the eukaryotic spliceosome results in formation of a complementary helix of seven base pairs with a single unpaired adenosine residue. The 2' OH of this adenosine, called the branch site, brings about nucleophilic attack at the pre-mRNA 5' splice site in the first step of splicing. Another feature of this pairing is the phylogenetic conservation of a pseudouridine (psi) residue in U2 snRNA nearly opposite the branch site. We show that the presence of this psi in the pre-mRNA branch-site helix of Saccharomyces cerevisiae induces a dramatically altered architectural landscape compared with that of its unmodified counterpart. The psi-induced structure places the nucleophile in an accessible position for the first step of splicing.
Pairing of a consensus sequence of the precursor (pre)-mRNA intron with a short region of the U2 small nuclear (sn)RNA during assembly of the eukaryotic spliceosome results in formation of a complementary helix of seven base pairs with a single unpaired adenosine residue. The 2' OH of this adenosine, called the branch site, brings about nucleophilic attack at the pre-mRNA 5' splice site in the first step of splicing. Another feature of this pairing is the phylogenetic conservation of a pseudouridine (psi) residue in U2 snRNA nearly opposite the branch site. We show that the presence of this psi in the pre-mRNA branch-site helix of Saccharomyces cerevisiae induces a dramatically altered architectural landscape compared with that of its unmodified counterpart. The psi-induced structure places the nucleophile in an accessible position for the first step of splicing.
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==About this Structure==
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Sculpting of the spliceosomal branch site recognition motif by a conserved pseudouridine.,Newby MI, Greenbaum NL Nat Struct Biol. 2002 Dec;9(12):958-65. PMID:12426583<ref>PMID:12426583</ref>
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1LMV is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LMV OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Sculpting of the spliceosomal branch site recognition motif by a conserved pseudouridine., Newby MI, Greenbaum NL, Nat Struct Biol. 2002 Dec;9(12):958-65. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12426583 12426583]
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</div>
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[[Category: Protein complex]]
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<div class="pdbe-citations 1lmv" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Greenbaum, N L.]]
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[[Category: Greenbaum NL]]
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[[Category: Newby, M I.]]
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[[Category: Newby MI]]
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[[Category: a-form helix]]
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[[Category: branch site]]
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[[Category: nmr]]
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[[Category: solution structure]]
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[[Category: u2 snrna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:04:46 2008''
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Current revision

Solution structure of the unmodified U2 snRNA-intron branch site helix from S. cerevisiae

PDB ID 1lmv

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