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| | ==Hypothetical protein PA2703 Pseudomonas aeruginosa PAO1== | | ==Hypothetical protein PA2703 Pseudomonas aeruginosa PAO1== |
| - | <StructureSection load='3stq' size='340' side='right' caption='[[3stq]], [[Resolution|resolution]] 2.28Å' scene=''> | + | <StructureSection load='3stq' size='340' side='right'caption='[[3stq]], [[Resolution|resolution]] 2.28Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3stq]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3STQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3STQ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3stq]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3STQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3STQ FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PA2703 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.284Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3stq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3stq OCA], [http://pdbe.org/3stq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3stq RCSB], [http://www.ebi.ac.uk/pdbsum/3stq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3stq ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3stq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3stq OCA], [https://pdbe.org/3stq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3stq RCSB], [https://www.ebi.ac.uk/pdbsum/3stq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3stq ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/TSI2_PSEAE TSI2_PSEAE] Immunity protein that plays a role in preventing early activation of toxin Tse2. Binds to a large surface of Tse2 and thereby occludes the active site to specifically inhibits Tse2.<ref>PMID:22310046</ref> <ref>PMID:22511866</ref> <ref>PMID:26749446</ref> |
| - | In the competition for niches in natural resources, Pseudomonas aeruginosa utilizes the type VI secretion system to inject the toxic protein effector Tse2 into bacteria on cell-cell contact. The cytoplasm toxin immunity protein Tsi2 can neutralize Tse2 by physical interaction with the toxin, providing essential protection from toxin activity. Except for orthologues in P. aeruginosa, Tsi2 antitoxin does not share detectable sequence homology with known proteins in public databases. The mechanism underlying toxin neutralization by Tsi2 remains unknown. We report here the crystal structure of Tsi2 at 2.28 A resolution. Our structural and biophysical analyses demonstrate that the antitoxin adopts a previously unobserved superhelical conformation. Tsi2 is highly thermostable in the absence of the toxin in solution. Tsi2 assembles a dimer with 2-fold rotational symmetry, similar to that observed in other toxin-antitoxin systems. Dimerization is essential for the stable folding of Tsi2.
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| - | Crystal structure of Pseudomonas aeruginosa Tsi2 reveals a stably folded superhelical antitoxin.,Zou T, Yao X, Qin B, Zhang M, Cai L, Shang W, Svergun DI, Wang M, Cui S, Jin Q J Mol Biol. 2012 Apr 6;417(4):351-61. Epub 2012 Jan 30. PMID:22310046<ref>PMID:22310046</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div> | + | |
| - | <div class="pdbe-citations 3stq" style="background-color:#fffaf0;"></div> | + | |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Cui, S]] | + | [[Category: Large Structures]] |
| - | [[Category: Jin, Q]] | + | [[Category: Pseudomonas aeruginosa]] |
| - | [[Category: Wang, M T]] | + | [[Category: Cui S]] |
| - | [[Category: Zou, T T]] | + | [[Category: Jin Q]] |
| - | [[Category: Coiled-coil]] | + | [[Category: Wang MT]] |
| - | [[Category: T6ss]] | + | [[Category: Zou TT]] |
| - | [[Category: Toxin]]
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| - | [[Category: Toxin immunity]]
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| - | [[Category: Toxin-antitoxin system]]
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| - | [[Category: Tsi2-tse2]]
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| Structural highlights
Function
TSI2_PSEAE Immunity protein that plays a role in preventing early activation of toxin Tse2. Binds to a large surface of Tse2 and thereby occludes the active site to specifically inhibits Tse2.[1] [2] [3]
References
- ↑ Zou T, Yao X, Qin B, Zhang M, Cai L, Shang W, Svergun DI, Wang M, Cui S, Jin Q. Crystal structure of Pseudomonas aeruginosa Tsi2 reveals a stably folded superhelical antitoxin. J Mol Biol. 2012 Apr 6;417(4):351-61. Epub 2012 Jan 30. PMID:22310046 doi:10.1016/j.jmb.2012.01.040
- ↑ Li M, Le Trong I, Carl MA, Larson ET, Chou S, De Leon JA, Dove SL, Stenkamp RE, Mougous JD. Structural Basis for Type VI Secretion Effector Recognition by a Cognate Immunity Protein. PLoS Pathog. 2012 Apr;8(4):e1002613. Epub 2012 Apr 12. PMID:22511866 doi:10.1371/journal.ppat.1002613
- ↑ Robb CS, Robb M, Nano FE, Boraston AB. The Structure of the Toxin and Type Six Secretion System Substrate Tse2 in Complex with Its Immunity Protein. Structure. 2015 Dec 28. pii: S0969-2126(15)00501-8. doi:, 10.1016/j.str.2015.11.012. PMID:26749446 doi:http://dx.doi.org/10.1016/j.str.2015.11.012
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