3tb6

<StructureSection load='3tb6' size='340' side='right' caption='[[3tb6]], [[Resolution|resolution]] 2.21&Aring;' scene=''>

<table><tr><td colspan='2'>[[3tb6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TB6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TB6 FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ARB:BETA-L-ARABINOSE'>ARB</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>

<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">araC, araR, BSU33970, yvbS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Bacillus globigii" Migula 1900])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tb6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tb6 OCA], [http://pdbe.org/3tb6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tb6 RCSB], [http://www.ebi.ac.uk/pdbsum/3tb6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tb6 ProSAT]</span></td></tr>

[[http://www.uniprot.org/uniprot/ARAR_BACSU ARAR_BACSU]] Transcriptional repressor of the arabinose utilization genes. Also regulates its own expression. Binds to two sequences within the promoters of the araABDLMNPQ-abfA operon and the araE gene, and to one sequence in the araR promoter.

In Bacillus subtilis, the arabinose repressor AraR negatively controls the expression of genes in the metabolic pathway of arabinose-containing polysaccharides. The protein is composed of two domains of different phylogenetic origin and function: an N-terminal DNA-binding domain belonging to the GntR family and a C-terminal effector-binding domain that shows similarity to members of the GalR/LacI family. The crystal structure of the C-terminal effector-binding domain of AraR in complex with the effector L-arabinose has been determined at 2.2 A resolution. The L-arabinose binding affinity was characterized by isothermal titration calorimetry and differential scanning fluorimetry; the K(d) value was 8.4 +/- 0.4 microM. The effect of L-arabinose on the protein oligomeric state was investigated in solution and detailed analysis of the crystal identified a dimer organization which is distinctive from that of other members of the GalR/LacI family.

 

== References ==

[[Category: Bacillus globigii migula 1900]]

[[Category: Prochazkova, K]]

[[Category: Rezacova, P]]

[[Category: Arabinose binding]]

[[Category: Transcription regulation]]