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3pty

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==Crystal structure of the C-terminal extracellular domain of Mycobacterium tuberculosis EmbC==
==Crystal structure of the C-terminal extracellular domain of Mycobacterium tuberculosis EmbC==
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<StructureSection load='3pty' size='340' side='right' caption='[[3pty]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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<StructureSection load='3pty' size='340' side='right'caption='[[3pty]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3pty]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PTY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PTY FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3pty]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PTY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PTY FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AFO:OCTYL+ALPHA-D-ARABINOFURANOSIDE'>AFO</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">embC, MT3900, MTCY13D12.27, Rv3793 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AFO:OCTYL+ALPHA-D-ARABINOFURANOSIDE'>AFO</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pty FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pty OCA], [http://pdbe.org/3pty PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3pty RCSB], [http://www.ebi.ac.uk/pdbsum/3pty PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3pty ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pty FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pty OCA], [https://pdbe.org/3pty PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pty RCSB], [https://www.ebi.ac.uk/pdbsum/3pty PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pty ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/EMBC_MYCTU EMBC_MYCTU]] Arabinosyl transferase responsible for the polymerization of arabinose into the arabinan of arabinogalactan.
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[https://www.uniprot.org/uniprot/EMBC_MYCTU EMBC_MYCTU] Arabinosyl transferase responsible for the polymerization of arabinose into the arabinan of arabinogalactan.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The d-arabinan-containing polymers arabinogalactan (AG) and lipoarabinomannan (LAM) are essential components of the unique cell envelope of the pathogen Mycobacterium tuberculosis. Biosynthesis of AG and LAM involves a series of membrane-embedded arabinofuranosyl (Araf) transferases whose structures are largely uncharacterised, despite the fact that several of them are pharmacological targets of ethambutol, a frontline drug in tuberculosis therapy. Herein, we present the crystal structure of the C-terminal hydrophilic domain of the ethambutol-sensitive Araf transferase M. tuberculosis EmbC, which is essential for LAM synthesis. The structure of the C-terminal domain of EmbC (EmbC(CT)) encompasses two sub-domains of different folds, of which subdomain II shows distinct similarity to lectin-like carbohydrate-binding modules (CBM). Co-crystallisation with a cell wall-derived di-arabinoside acceptor analogue and structural comparison with ligand-bound CBMs suggest that EmbC(CT) contains two separate carbohydrate binding sites, associated with subdomains I and II, respectively. Single-residue substitution of conserved tryptophan residues (Trp868, Trp985) at these respective sites inhibited EmbC-catalysed extension of LAM. The same substitutions differentially abrogated binding of di- and penta-arabinofuranoside acceptor analogues to EmbC(CT), linking the loss of activity to compromised acceptor substrate binding, indicating the presence of two separate carbohydrate binding sites, and demonstrating that subdomain II indeed functions as a carbohydrate-binding module. This work provides the first step towards unravelling the structure and function of a GT-C-type glycosyltransferase that is essential in M. tuberculosis.
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The C-Terminal Domain of the Arabinosyltransferase Mycobacterium tuberculosis EmbC Is a Lectin-Like Carbohydrate Binding Module.,Alderwick LJ, Lloyd GS, Ghadbane H, May JW, Bhatt A, Eggeling L, Futterer K, Besra GS PLoS Pathog. 2011 Feb;7(2):e1001299. Epub 2011 Feb 24. PMID:21383969<ref>PMID:21383969</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3pty" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Alderwick, L J]]
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[[Category: Large Structures]]
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[[Category: Besra, G S]]
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[[Category: Mycobacterium tuberculosis]]
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[[Category: Futterer, K]]
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[[Category: Alderwick LJ]]
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[[Category: Beta-sandwich]]
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[[Category: Besra GS]]
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[[Category: Carbohydrate]]
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[[Category: Futterer K]]
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[[Category: Carbohydrate binding]]
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[[Category: Transferase]]
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Current revision

Crystal structure of the C-terminal extracellular domain of Mycobacterium tuberculosis EmbC

PDB ID 3pty

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