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| | ==Crystal Structure of the vibrio cholerae secreted colonization factor TcpF== | | ==Crystal Structure of the vibrio cholerae secreted colonization factor TcpF== |
| - | <StructureSection load='3oc5' size='340' side='right' caption='[[3oc5]], [[Resolution|resolution]] 2.40Å' scene=''> | + | <StructureSection load='3oc5' size='340' side='right'caption='[[3oc5]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3oc5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OC5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OC5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3oc5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OC5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OC5 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3oc8|3oc8]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tcpF, VC_0837 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3oc5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oc5 OCA], [https://pdbe.org/3oc5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3oc5 RCSB], [https://www.ebi.ac.uk/pdbsum/3oc5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3oc5 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3oc5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oc5 OCA], [http://pdbe.org/3oc5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3oc5 RCSB], [http://www.ebi.ac.uk/pdbsum/3oc5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3oc5 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TCPF_VIBCH TCPF_VIBCH]] Involved in TCP pilus biogenesis. May be a channel protein. | + | [https://www.uniprot.org/uniprot/TCPF_VIBCH TCPF_VIBCH] Involved in TCP pilus biogenesis. May be a channel protein. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| | *[[Colonization factor antigen|Colonization factor antigen]] | | *[[Colonization factor antigen|Colonization factor antigen]] |
| - | *[[Vibrio cholerae colonization factor TcpF|Vibrio cholerae colonization factor TcpF]] | |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillo virgola del koch trevisan 1884]] | + | [[Category: Large Structures]] |
| - | [[Category: Craig, L]] | + | [[Category: Vibrio cholerae]] |
| - | [[Category: Kolappan, S]] | + | [[Category: Craig L]] |
| - | [[Category: Yuen, A S.W]] | + | [[Category: Kolappan S]] |
| - | [[Category: Cell adhesion]] | + | [[Category: Yuen ASW]] |
| - | [[Category: Multidomain protein]]
| + | |
| Structural highlights
Function
TCPF_VIBCH Involved in TCP pilus biogenesis. May be a channel protein.
Publication Abstract from PubMed
Vibrio cholerae relies on two main virulence factors-toxin-coregulated pilus (TCP) and cholera toxin-to cause the gastrointestinal disease cholera. TCP is a type IV pilus that mediates bacterial autoagglutination and colonization of the intestine. TCP is encoded by the tcp operon, which also encodes TcpF, a protein of unknown function that is secreted by V. cholerae in a TCP-dependent manner. Although TcpF is not required for TCP biogenesis, a tcpF mutant has a colonization defect in the infant mouse cholera model that is as severe as a pilus mutant. Furthermore, TcpF antisera protect against V. cholerae infection. TcpF has no apparent sequence homology to any known protein. Here, we report the de novo X-ray crystal structure of TcpF and the identification of an epitope that is critical for its function as a colonization factor. A monoclonal antibody recognizing this epitope is protective against V. cholerae challenge and adds to the protection provided by an anti-TcpA antibody. These data suggest that TcpF has a novel function in V. cholerae colonization and define a region crucial for this function.
Crystal Structure of the Vibrio cholerae Colonization Factor TcpF and Identification of a Functional Immunogenic Site.,Megli CJ, Yuen AS, Kolappan S, Richardson MR, Dharmasena MN, Krebs SJ, Taylor RK, Craig L J Mol Biol. 2011 Mar 31. PMID:21440558[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Megli CJ, Yuen AS, Kolappan S, Richardson MR, Dharmasena MN, Krebs SJ, Taylor RK, Craig L. Crystal Structure of the Vibrio cholerae Colonization Factor TcpF and Identification of a Functional Immunogenic Site. J Mol Biol. 2011 Mar 31. PMID:21440558 doi:10.1016/j.jmb.2011.03.027
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