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| | ==Crystal Structure of the E.coli DhaR(N)-DhaL complex== | | ==Crystal Structure of the E.coli DhaR(N)-DhaL complex== |
| - | <StructureSection load='4lrz' size='340' side='right' caption='[[4lrz]], [[Resolution|resolution]] 2.32Å' scene=''> | + | <StructureSection load='4lrz' size='340' side='right'caption='[[4lrz]], [[Resolution|resolution]] 2.32Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4lrz]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LRZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LRZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4lrz]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LRZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LRZ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.32Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4lrx|4lrx]], [[4lry|4lry]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">b1199, dhaL, dhaR, JW5186, ycgS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI]), b1201, dhaL, dhaR, JW5188, ycgU ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lrz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lrz OCA], [https://pdbe.org/4lrz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lrz RCSB], [https://www.ebi.ac.uk/pdbsum/4lrz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lrz ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lrz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lrz OCA], [http://pdbe.org/4lrz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lrz RCSB], [http://www.ebi.ac.uk/pdbsum/4lrz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lrz ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/DHAL_ECOLI DHAL_ECOLI]] ADP-binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. DhaL-ADP receives a phosphoryl group from DhaM and transmits it to dihydroxyacetone. DhaL-ADP acts also as a coactivator by binding to the sensor domain of DhaR. DhaL-ATP is inactive. [[http://www.uniprot.org/uniprot/DHAR_ECOLI DHAR_ECOLI]] Positively regulates the dhaKLM operon from a sigma-70 promoter. Represses its own synthesis. | + | [https://www.uniprot.org/uniprot/DHAL_ECOLI DHAL_ECOLI] ADP-binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. DhaL-ADP receives a phosphoryl group from DhaM and transmits it to dihydroxyacetone. DhaL-ADP acts also as a coactivator by binding to the sensor domain of DhaR. DhaL-ATP is inactive. |
| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Escherichia coli dihydroxyacetone (Dha) kinase consists of two subunits, DhaK and DhaL. Transcription of dha operon is regulated by the DhaR transcription factor and its action is under control of the kinase subunits. DhaR is activated by interaction with DhaL while it is repressed by DhaK. We have determined the structures of DhaK and DhaL bound to the tandem GAF-like and PAS domains of the DhaR, providing an architectural model for how GAF/PAS tandem domains work together in binding protein partners. The structures reveal a mechanism of opposite transcriptional regulation by the DhaK and DhaL subunits. The kinase subunits interface with DhaR through surfaces that partially overlap with their active sites, allowing sensing of ATP- versus ADP-loaded DhaL subunit and also precluding a ternary complex between DhaK-DhaL and DhaR. The rotation of helices within the DhaR coiled-coil linker upon DhaL binding provides the mechanism for transmitting the binding signal from the GAF/PAS domains to the C-terminal DNA-binding domain.
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| - | Coiled-Coil Helix Rotation Selects Repressing or Activating State of Transcriptional Regulator DhaR.,Shi R, McDonald L, Cygler M, Ekiel I Structure. 2014 Jan 14. pii: S0969-2126(13)00471-1. doi:, 10.1016/j.str.2013.11.012. PMID:24440518<ref>PMID:24440518</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 4lrz" style="background-color:#fffaf0;"></div>
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| - | == References ==
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| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ecoli]] | + | [[Category: Escherichia coli K-12]] |
| - | [[Category: Cygler, M]] | + | [[Category: Large Structures]] |
| - | [[Category: Ekiel, I]] | + | [[Category: Cygler M]] |
| - | [[Category: McDonald, L]] | + | [[Category: Ekiel I]] |
| - | [[Category: Shi, R]] | + | [[Category: McDonald L]] |
| - | [[Category: Coiled-coil]]
| + | [[Category: Shi R]] |
| - | [[Category: Gaf]]
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| - | [[Category: Helix rotation]]
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| - | [[Category: Pa]]
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| - | [[Category: Transcriptional regulation complex]]
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| - | [[Category: Transferase-transcription regulator complex]]
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