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4hvk

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==Crystal structure and functional studies of an unusual L-cysteine desulfurase from Archaeoglobus fulgidus.==
==Crystal structure and functional studies of an unusual L-cysteine desulfurase from Archaeoglobus fulgidus.==
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<StructureSection load='4hvk' size='340' side='right' caption='[[4hvk]], [[Resolution|resolution]] 1.43&Aring;' scene=''>
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<StructureSection load='4hvk' size='340' side='right'caption='[[4hvk]], [[Resolution|resolution]] 1.43&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4hvk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arcfu Arcfu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HVK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HVK FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4hvk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HVK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HVK FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PMP:4-DEOXY-4-AMINOPYRIDOXAL-5-PHOSPHATE'>PMP</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.43&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4eb5|4eb5]], [[4eb7|4eb7]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PMP:4-DEOXY-4-AMINOPYRIDOXAL-5-PHOSPHATE'>PMP</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AF0564, AF_0564, iscS2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224325 ARCFU])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hvk OCA], [https://pdbe.org/4hvk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hvk RCSB], [https://www.ebi.ac.uk/pdbsum/4hvk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hvk ProSAT]</span></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cysteine_desulfurase Cysteine desulfurase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.1.7 2.8.1.7] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hvk OCA], [http://pdbe.org/4hvk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hvk RCSB], [http://www.ebi.ac.uk/pdbsum/4hvk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hvk ProSAT]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/ISCS2_ARCFU ISCS2_ARCFU]] Catalyzes the removal of elemental sulfur from cysteine to produce alanine (By similarity).
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[https://www.uniprot.org/uniprot/ISCS2_ARCFU ISCS2_ARCFU] Catalyzes the removal of elemental sulfur from cysteine to produce alanine (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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l-Cysteine desulfurase IscS and scaffold IscU proteins are universally involved in Fe/S cluster synthesis. The Archaeoglobus fulgidus (Af) genome encodes proteins having a high degree of primary structure similarity to IscS and IscU from other organisms. However, AfIscS is unusual because it lacks the active site lysine residue that normally forms an internal Schiff base with pyridoxal-phosphate (PLP) and serves as a base during catalysis. Our as-isolated recombinant AfIscS contains pyridoxamine phosphate (PMP) instead of the expected PLP and lacks desulfurase activity. We have solved its structure to 1.43 A resolution and found that PMP binds non-covalently at the PLP site of the enzyme and displays significant disorder. However, the previously reported structure of recombinant Af(IscU-D35A-IscS)(2) contains an in vivo generated [Fe(2)S(2)] species within AfIscU and the question arises as to how its sulfides were generated. Here, we report that adding PLP to AfIscS produces an enzyme that displays in vitrol-cysteine desulfurase activity mediating the synthesis of a stable holo Af(IscU-D35A-IscS) complex.
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Crystal structure and functional studies of an unusual l-cysteine desulfurase from Archaeoglobus fulgidus.,Yamanaka Y, Zeppieri L, Nicolet Y, Marinoni EN, de Oliveira JS, Odaka M, Dean DR, Fontecilla-Camps JC Dalton Trans. 2012 Nov 19. PMID:23160436<ref>PMID:23160436</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4hvk" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Arcfu]]
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[[Category: Archaeoglobus fulgidus DSM 4304]]
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[[Category: Cysteine desulfurase]]
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[[Category: Large Structures]]
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[[Category: Dean, D R]]
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[[Category: Dean DR]]
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[[Category: Fontecilla-Camps, J C]]
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[[Category: Fontecilla-Camps JC]]
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[[Category: Marinoni, E N]]
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[[Category: Marinoni EN]]
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[[Category: Masafumi, O]]
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[[Category: Masafumi O]]
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[[Category: Nicolet, Y]]
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[[Category: Nicolet Y]]
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[[Category: Oliveira, J S.de]]
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[[Category: Yamanaka Y]]
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[[Category: Yamanaka, Y]]
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[[Category: Zeppieri L]]
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[[Category: Zeppieri, L]]
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[[Category: De Oliveira JS]]
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[[Category: Transferase]]
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[[Category: Transferase and isc]]
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Current revision

Crystal structure and functional studies of an unusual L-cysteine desulfurase from Archaeoglobus fulgidus.

PDB ID 4hvk

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